Lehmann D, Müller H
Int J Androl. 1987 Feb;10(1):163-8. doi: 10.1111/j.1365-2605.1987.tb00178.x.
Immune response in a patient with an in-situ seminoma of the testis was studied. Immunohistochemical examination of the testicular biopsy demonstrated remarkable intracellular and membraneous accumulation of IgG antibodies in the atypical spermatogonia. Using FITC-conjugated Concanavalin A, an abnormally high binding of Concanavalin A was detected in the transformed cells. Circulating antibodies directed against an antigenic determinant (60 kD) which is expressed on normal human spermatozoa and lymphocytes were found only in the patient's serum and not in 500 control sera. The role and possible diagnostic value of this autoimmune reactivity in testicular malignancies are discussed.
对一名患有睾丸原位精原细胞瘤患者的免疫反应进行了研究。对睾丸活检组织进行免疫组化检查显示，在非典型精原细胞中存在显著的IgG抗体细胞内和膜性积聚。使用异硫氰酸荧光素（FITC）偶联的刀豆球蛋白A，在转化细胞中检测到刀豆球蛋白A的异常高结合。仅在患者血清中发现了针对正常人类精子和淋巴细胞上表达的一种抗原决定簇（60kD）的循环抗体，而在500份对照血清中未发现。讨论了这种自身免疫反应在睾丸恶性肿瘤中的作用及可能的诊断价值。
