Lehmann D, Temminck B, Litmanen K, Leibundgut B, Hadziselimovic F, Müller H
Cancer. 1986 Nov 1;58(9):2013-7. doi: 10.1002/1097-0142(19861101)58:9<2013::aid-cncr2820580910>3.0.co;2-o.
Routine examination of a testis biopsy from a patient with oligospermia and a large varicocele in his left testicle and who was childless for over 2 years showed carcinoma in situ (CIS) with atypic spermatogonia in his right testicle. Immunohistochemical investigations of this tissue revealed large intracellular deposits of immunoglobulin G (IgG) restricted to the atypical cells. By blot-immunobinding test of the patient's serum, circulating antibodies against a molecule with an approximate molecular weight of 60 kD were found. Such antibodies were not found in a total of over 500 infertile patients and fertile controls examined. This molecule was extracted from pooled normal human sperms by trypsin digestion and eluted out of a sodium dodecyl-sulphate polyacrylamide gel (SDS-PAGE). This elute is currently used for raising monoclonal antibodies. An analysis of the direct chromosome preparations from the testis biopsy showed both numerical and structural chromosomal aberrations. This might indicate that the atypical cells have already been transformed and hence could be considered as malignant.
对一名患有少精子症且左侧睾丸有大的精索静脉曲张、两年多未育的患者进行睾丸活检的常规检查发现，其右侧睾丸存在原位癌（CIS）及非典型精原细胞。对该组织进行免疫组织化学研究显示，免疫球蛋白G（IgG）大量细胞内沉积局限于非典型细胞。通过对患者血清进行印迹免疫结合试验，发现了针对一种分子量约为60 kD分子的循环抗体。在总共500多名接受检查的不育患者和生育对照组中未发现此类抗体。该分子通过胰蛋白酶消化从正常人精子库中提取，并从十二烷基硫酸钠聚丙烯酰胺凝胶（SDS-PAGE）中洗脱出来。这种洗脱物目前用于制备单克隆抗体。对睾丸活检的直接染色体标本分析显示存在染色体数目和结构异常。这可能表明非典型细胞已经发生转化，因此可被视为恶性细胞。
