Department of General Surgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121000, PR China.
Out-patient Department, Jinzhou Central Hospital, Jinzhou, Liaoning, 121000, PR China.
Mol Cell Probes. 2020 Dec;54:101667. doi: 10.1016/j.mcp.2020.101667. Epub 2020 Sep 16.
Most cancer cells preferentially metabolize glucose by glycolysis rather than oxidative phosphorylation to proliferate efficiently. LncRNAs have been proposed as crucial regulators in pathophysiological processes including cell growth, apoptosis and glucose metabolism. However, little is known regarding the specific role of LINC00346 in regulating glucose metabolism in breast cancer. LINC00346 and miR-148a/b expression in breast cancer cells was detected by qRT-PCR. The relationships between LINC00346, glucose transporter 1 (GLUT1) and miR-148a/b in breast cancer cells were explored by luciferase reporter assay. Cell proliferation and apoptosis were evaluated by CCK-8 and flow cytometry analysis, respectively. Glycolysis was detected by measuring the glucose uptake and lactate production. Results showed that LINC00346 was over-expressed while miR-148a/b was low-expressed in breast cancer cells. miR-148a/b were direct targets of LINC00346 in breast cancer cells. LINC00346 knockdown inhibited cell proliferation and glycolysis, and induced apoptosis by upregulating miR-148a/b in breast cancer cells. Furthermore, we found that LINC00346 knockdown repressed GLUT1 expression in breast cancer cells by upregulating miR-148a/b. In conclusion, LINC00346 knockdown suppressed breast cancer cell glycolysis by upregulating miR-148a/b and repressing GLUT1 expression.
大多数癌细胞通过糖酵解优先代谢葡萄糖，而不是通过氧化磷酸化来高效增殖。lncRNAs 被认为是包括细胞生长、凋亡和葡萄糖代谢在内的病理生理过程中的关键调节因子。然而，关于 LINC00346 在调节乳腺癌中葡萄糖代谢的具体作用知之甚少。通过 qRT-PCR 检测乳腺癌细胞中 LINC00346 和 miR-148a/b 的表达。通过荧光素酶报告基因检测探讨乳腺癌细胞中 LINC00346、葡萄糖转运蛋白 1 (GLUT1) 和 miR-148a/b 之间的关系。通过 CCK-8 和流式细胞术分析分别评估细胞增殖和凋亡。通过测量葡萄糖摄取和乳酸生成来检测糖酵解。结果表明，LINC00346 在乳腺癌细胞中过表达，而 miR-148a/b 低表达。miR-148a/b 是乳腺癌细胞中 LINC00346 的直接靶标。LINC00346 敲低通过上调乳腺癌细胞中的 miR-148a/b 抑制细胞增殖和糖酵解，并诱导细胞凋亡。此外，我们发现 LINC00346 敲低通过上调 miR-148a/b 抑制乳腺癌细胞中 GLUT1 的表达。总之，LINC00346 敲低通过上调 miR-148a/b 并抑制 GLUT1 的表达抑制乳腺癌细胞的糖酵解。
