Belekov Ermek, Kholikov Khomidkhodza, Cooper Lauren, Banga Simran, Er Ali O
Western Kentucky University, Department of Physics and Astronomy, Bowling Green, KY, 42101, United States.
Western Kentucky University, Department of Biology, Bowling Green, KY, 42101, United States.
Photodiagnosis Photodyn Ther. 2020 Dec;32:102012. doi: 10.1016/j.pdpdt.2020.102012. Epub 2020 Sep 15.
Photosensitizing agents are the cornerstone of Photodynamic Therapy (PDT). They play an essential role in deactivation process of multidrug resistant pathogens and tumor treatments. In this work, we studied a photosensitizing agent made from mixture of Silver Nanoparticles (Ag NPs) and Methylene Blue (MB) which possess improved important characteristics like high photostability and high singlet oxygen yield. Ag NPs were synthesized by pulsed laser ablation technique in different aqueous solutions like polyvinylpyrrolidone (PVP), citrate and Deionized (DI) water. The synthesized AgNPs were characterized in depth using with transmission electron microscopy (TEM), UV-vis (UV-vis), and photoluminescence (PL) spectra. These Ag NPs were combined with MB and used to eradicate the Gram-negative bacteria, Escherichia coli (E. coli), and Gram-positive bacteria, Staphylococcus aureus (S. aureus). MB and Ag NPs mixture was found to possess higher antimicrobial activity and thus were more effective in killing both Gram -positive and Gram-negative bacteria in comparison to individual exposure of MB and Ag NPs. Additionally, the antimicrobial effects varied with respect to the size of nanoparticles as well as the medium used for their synthesis. The data from this study supports the potential use of the proposed method in PDT where standard photosensitizers have limitations.
光敏剂是光动力疗法（PDT）的基石。它们在多药耐药病原体的失活过程和肿瘤治疗中起着至关重要的作用。在这项工作中，我们研究了一种由银纳米颗粒（Ag NPs）和亚甲蓝（MB）混合制成的光敏剂，其具有高光稳定性和高单线态氧产率等重要特性。通过脉冲激光烧蚀技术在聚乙烯吡咯烷酮（PVP）、柠檬酸盐和去离子水（DI）水等不同水溶液中合成了Ag NPs。使用透射电子显微镜（TEM）、紫外可见光谱（UV-vis）和光致发光（PL）光谱对合成的Ag NPs进行了深入表征。将这些Ag NPs与MB结合，用于根除革兰氏阴性菌大肠杆菌（E. coli）和革兰氏阳性菌金黄色葡萄球菌（S. aureus）。发现MB和Ag NPs的混合物具有更高的抗菌活性，因此与单独使用MB和Ag NPs相比，在杀死革兰氏阳性菌和革兰氏阴性菌方面更有效。此外，抗菌效果因纳米颗粒的大小以及用于合成它们的介质而异。这项研究的数据支持了所提出的方法在标准光敏剂存在局限性的光动力疗法中的潜在应用。
