International Program in Hazardous Substance and Environmental Management, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand; Research Program on Remediation Technologies for Petroleum Contamination, Center of Excellence on Hazardous Substance Management (HSM), Chulalongkorn University, Bangkok, Thailand.
Excellent Center of Waste Utilization and Management, Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok, Thailand; Biochemical Engineering and Systems Biology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at KMUTT, Bangkok, Thailand.
J Hazard Mater. 2020 Dec 5;400:123291. doi: 10.1016/j.jhazmat.2020.123291. Epub 2020 Jun 25.
Benzothiazoles especially 2-mercaptobenzothiazole (2-MBT) in rubber industrial wastewater can be released into the environment. They can cause adverse health impacts. This study aimed to obtain efficient 2-MBT-degrading bacteria for wastewater application. The bacterial consortia were enriched by incubating rubber wastewater sludge in a medium containing 2-MBT for 28 days. Stepwise acclimatization was conducted with increasing 2-MBT concentrations from 50 to 200 mg L in nitrogen-containing medium for 76 days. The process significantly increased the bacterial number and changed the dominant populations. Among these consortia, the EN consortium from benzothiazole-containing sludge had the highest specific 2-MBT biodegradation rate of 5.2 ± 0.5 mg L day mg protein and could degrade up to 300 mg L 2-MBT. From 16S rRNA gene analysis, Pseudomonas was the dominant genus at approximately 70 % of the total population. Stenotrophomonas was the second most abundant populations and have never been reported for 2-MBT biodegradation. The EN consortium removed 65-79 % and 90-93 % of 112 mg L 2-MBT and ∼4000 mg L COD in rubber wastewater, respectively, which were significantly higher than the values of natural attenuation. Therefore, the EN consortium could be an ideal inoculum for the post-treatment of benzothiazoles in rubber industrial wastewater.
苯并噻唑类物质，尤其是橡胶工业废水中的 2-巯基苯并噻唑（2-MBT），可能会释放到环境中。它们可能会对健康造成不良影响。本研究旨在获得高效的 2-MBT 降解菌，以应用于废水处理。通过在含有 2-MBT 的培养基中培养橡胶废水污泥 28 天，富集细菌菌群。通过在含氮培养基中逐步驯化，2-MBT 浓度从 50 增加到 200mg/L，历时 76 天。该过程显著增加了细菌数量，并改变了优势种群。在这些菌群中，来自含苯并噻唑污泥的 EN 菌群对 2-MBT 的特定生物降解率最高，为 5.2±0.5mg L day mg 蛋白，可降解高达 300mg/L 的 2-MBT。通过 16S rRNA 基因分析，假单胞菌约占总种群的 70%，是优势属。寡养单胞菌是第二丰富的种群，以前从未报道过其具有 2-MBT 生物降解能力。EN 菌群分别去除了橡胶废水中 112mg/L 2-MBT 和约 4000mg/L COD 的 65-79%和 90-93%，明显高于自然衰减的值。因此，EN 菌群可以作为橡胶工业废水中苯并噻唑后处理的理想接种物。
