Singha L Paikhomba, Shukla Pratyoosh
Enzyme Technology and Protein Bioinformatics Laboratory, School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
Bioprocess Biosyst Eng. 2023 Mar;46(3):323-339. doi: 10.1007/s00449-022-02777-x. Epub 2022 Aug 27.
Axenic microbial applications in the open environment are unrealistic and may not be always practically viable. Therefore, it is important to use mixed microbial cultures and their interactions with the microbiome in the targeted ecosystem to perform robust functions towards their sustainability in harsh environmental conditions. Emerging pollutants like phthalates and hydrocarbons that are toxic to several aquatic and terrestrial life forms in the water bodies and lands are an alarming situation. The present review explores the possibility of devising an inclusive eco-friendly strategy like microbiome engineering which proves to be a unique and crucial technology involving the power of microbial communication through quorum sensing. This review discusses the interspecies and intra-species communications between different microbial groups with their respective environments. Moreover, this review also envisages the efforts for designing the next level of microbiome-host engineering concept (MHEC). The focus of the review also extended toward using omics and metabolic network analysis-based tools for effective microbiome engineering. These approaches might be quite helpful in the future to understand such microbial interactions but it will be challenging to implement in the real environment to get the desired functions. Finally, the review also discusses multiple approaches for the bioremediation of toxic chemicals from the soil environment.
在开放环境中应用无菌微生物是不现实的，而且在实际中可能并不总是可行的。因此，利用混合微生物培养物及其与目标生态系统中微生物群落的相互作用，以便在恶劣环境条件下实现强大功能以维持其可持续性，这一点很重要。像邻苯二甲酸盐和碳氢化合物这样的新兴污染物，对水体和陆地中的多种水生和陆地生物有毒，这是一个令人担忧的情况。本综述探讨了设计一种包容性的生态友好策略（如微生物群落工程）的可能性，事实证明这是一项独特且关键的技术，它涉及通过群体感应实现微生物通讯的能力。本综述讨论了不同微生物群体之间及其与各自环境的种间和种内通讯。此外，本综述还设想了设计下一代微生物群落-宿主工程概念（MHEC）的努力。综述的重点还扩展到使用基于组学和代谢网络分析的工具进行有效的微生物群落工程。这些方法在未来可能有助于理解此类微生物相互作用，但要在实际环境中实施以获得所需功能将具有挑战性。最后，综述还讨论了从土壤环境中生物修复有毒化学物质的多种方法。
