Environmental Technology Division, Central Leather Research Institute, Adyar, Chennai 600 020, Tamil Nadu, India.
Biochemistry Laboratory, Central Leather Research Institute, Chennai, 600 020, Tamil Nadu, India.
J Hazard Mater. 2017 Jan 5;321:773-781. doi: 10.1016/j.jhazmat.2016.09.061. Epub 2016 Oct 3.
Microaerobic degradation of 2-Mercaptobenzothiazole (2-MBT) was investigated using an isolated bacterial strain CSMB1. It was identified as Alcaligenes sp. MH146 by genomic analysis. The isolate degraded 50mg/L concentration of 2-MBT which was measured in terms of Total organic carbon (TOC) (700mg/L). A maximum degradation of 86% with a residual TOC concentration of 101mg/L was obtained after 72h, with the biomass growth of 290mg/L. The presence of specific activity of catechol 2, 3 oxygenase was observed in all the tested derivatives of benzothiazoles and the benzene ring opening was observed through meta cleavage. By analyzing the 72h incubated culture supernatant, 2-MBT, and all its biotransformed products were degraded into polar compounds. With the analytical results obtained, a possible microaerobic degradative pathway was proposed and illustrated for 2-MBT. It is concluded that microaerophilic isolate CSMB1 was able to degrade 2-MBT and its intermediates by utilizing them as sole carbon and energy.
采用分离得到的一株细菌 CSMB1 对 2-巯基苯并噻唑（2-MBT）的微好氧降解进行了研究。通过基因组分析，将该分离菌鉴定为产碱杆菌 MH146。该菌在总有机碳（TOC）（700mg/L）的基础上，降解了 50mg/L 浓度的 2-MBT。72h 后，生物量增长了 290mg/L，获得了 86%的最大降解率，TOC 残留浓度为 101mg/L。在所有测试的苯并噻唑衍生物中都观察到儿茶酚 2,3-双加氧酶的比活性，并且通过间位裂解观察到苯环的开环。通过分析 72h 孵育培养上清液，2-MBT 及其所有生物转化产物都被降解成极性化合物。根据获得的分析结果，提出并说明了 2-MBT 的可能的微好氧降解途径。结论是，微好氧分离株 CSMB1 能够利用 2-MBT 及其中间产物作为唯一的碳源和能源进行降解。
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