大肠杆菌K-12中酪氨酸和苯丙氨酸生物合成的调控：tyrR基因产物的特性
Regulation of tyrosine and phenylalanine biosynthesis in Escherichia coli K-12: properties of the tyrR gene product.
作者信息
Camakaris H, Pittard J
出版信息
J Bacteriol. 1973 Sep;115(3):1135-44. doi: 10.1128/jb.115.3.1135-1144.1973.
Abstract
A spontaneous amber tyrR mutant has been isolated in which constitutive synthesis of 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (tyr) and DAHP synthetase (phe) is suppressible by supC(-), supD(-), supF(-) and supU(-). This finding suggests the tyrR gene product is a protein. Derepression of DAHP synthetase (phe) in this and in seven other spontaneous tyrR mutants and in four Mu-1-induced tyrR mutants provides further evidence for the involvement of the tyrR gene product in phenylalanine biosynthesis. Evidence that the tyrR product is a component of repressor, rather than an enzyme involved in its synthesis or modification, comes from a study of a temperature-sensitive tyrR mutant. This mutant is of the thermolabile type, since derepression occurs rapidly and in the presence and absence of growth.
摘要
已分离出一种自发的琥珀色tyrR突变体，其中3-脱氧-D-阿拉伯庚酮糖酸7-磷酸（DAHP）合成酶（tyr）和DAHP合成酶（phe）的组成型合成可被supC（-）、supD（-）、supF（-）和supU（-）抑制。这一发现表明tyrR基因产物是一种蛋白质。在该突变体以及其他七个自发tyrR突变体和四个Mu-1诱导的tyrR突变体中，DAHP合成酶（phe）的去阻遏为tyrR基因产物参与苯丙氨酸生物合成提供了进一步证据。tyrR产物是阻遏物的一个组成部分，而不是参与其合成或修饰的酶，这一证据来自对一个温度敏感型tyrR突变体的研究。该突变体属于热不稳定型，因为去阻遏在有生长和无生长的情况下都迅速发生。
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Regulation of tyrosine and phenylalanine biosynthesis in Escherichia coli K-12: properties of the tyrR gene product.大肠杆菌K-12中酪氨酸和苯丙氨酸生物合成的调控：tyrR基因产物的特性
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