Hort Vincent, Arnich Nathalie, Guérin Thierry, Lavison-Bompard Gwenaëlle, Nicolas Marina
Université Paris-Est, ANSES, Laboratory for Food Safety, F-94701 Maisons-Alfort, France.
ANSES, Risk Assessment Directorate, F-94701 Maisons-Alfort, France.
Toxins (Basel). 2020 Sep 16;12(9):599. doi: 10.3390/toxins12090599.
In 2015, tetrodotoxins (TTXs) were considered a potential threat in Europe since several studies had shown the presence of these toxins in European bivalve molluscs. In this study, we investigated the occurrence of TTXs in 127 bivalve samples (mussels and oysters) and in 66 gastropod samples (whelks) collected all along the French mainland coasts in 2017 and 2018. Analyses were carried out after optimization and in-house validation of a performing hydrophilic interaction liquid chromatography associated with tandem mass spectrometry (HILIC-MS/MS) method. The concentration set by European Food Safety Authority (EFSA) not expected to result in adverse effects (44 µg TTX equivalent/kg) was never exceeded, but TTX was detected in three mussel samples and one whelk sample (1.7-11.2 µg/kg). The tissue distribution of TTX in this whelk sample showed higher concentrations in the digestive gland, stomach and gonads (7.4 µg TTX/kg) than in the rest of the whelk tissues (below the limit of detection of 1.7 µg TTX/kg). This is the first study to report the detection of TTX in French molluscs.
2015年，河豚毒素（TTXs）在欧洲被视为一种潜在威胁，因为多项研究表明这些毒素存在于欧洲双壳贝类软体动物中。在本研究中，我们调查了2017年和2018年在法国大陆沿岸采集的127份双壳类样本（贻贝和牡蛎）以及66份腹足类样本（蛾螺）中TTXs的存在情况。分析是在优化并经内部验证的高效亲水相互作用液相色谱-串联质谱联用（HILIC-MS/MS）方法之后进行的。欧洲食品安全局（EFSA）设定的预计不会产生不良影响的浓度（44微克TTX当量/千克）从未被超过，但在3份贻贝样本和1份蛾螺样本中检测到了TTX（1.7 - 11.2微克/千克）。该蛾螺样本中TTX的组织分布显示，消化腺、胃和性腺中的浓度（7.4微克TTX/千克）高于蛾螺其他组织中的浓度（低于检测限1.7微克TTX/千克）。这是首次报道在法国软体动物中检测到TTX的研究。
