Williams G L, Seaton B, McCarthy D
J Bacteriol. 1987 Jun;169(6):2643-9. doi: 10.1128/jb.169.6.2643-2649.1987.
Haemophilus influenzae was found to produce a DNA polymerase that was similar to polymerase I of Escherichia coli. E. coli polA mutants were used as backgrounds for the selection of H. influenzae polA suppressor genes. Six different H. influenzae fragments were isolated that could suppress E. coli polA mutations. None of the suppressors appeared to encode the H. influenzae equivalent of the E. coli polA gene. One type of clone, represented by pGW41, caused a polymerase I activity to appear in a suppressed polA1 mutant. Plasmids from the pGW41 class contained two genes (pol-2 and pol-3) that were both required for polA suppression. Mutated nonsuppressing derivatives of the pGW41 class were used to create H. influenzae mutants that were deficient in polymerase I.
发现流感嗜血杆菌可产生一种与大肠杆菌聚合酶I相似的DNA聚合酶。以大肠杆菌polA突变体作为背景来筛选流感嗜血杆菌polA抑制基因。分离出了六个不同的流感嗜血杆菌片段，它们能够抑制大肠杆菌polA突变。这些抑制子似乎都不编码与大肠杆菌polA基因相对应的流感嗜血杆菌基因。一种以pGW41为代表的克隆类型，可使聚合酶I活性出现在被抑制的polA1突变体中。来自pGW41类的质粒含有两个基因（pol - 2和pol - 3），这两个基因对于polA抑制都是必需的。pGW41类的突变非抑制衍生物被用于构建聚合酶I缺陷的流感嗜血杆菌突变体。
