Servos S, Khan S, Maskell D
Department of Biochemistry, Imperial College of Science Technology and Medicine, London, UK.
Gene. 1996 Oct 10;175(1-2):137-41. doi: 10.1016/0378-1119(96)00139-4.
Genes similar to Escherichia coli lpx genes (encoding enzymes required for the biosynthesis of lipid A) have been cloned from Haemophilus influenzae type b using a hybridisation-based strategy. The derived amino acid sequences are highly homologous to their E. coli counterparts. The genes appear in the same order in both E. coli and H. influenzae, but the intergenic regions differ. H. influenzae lpxA and lpxB have been expressed in E. coli minicells and they encode proteins of the predicted sizes. Both H. influenzae lpxA and lpxB are able to complement temperature-sensitive mutants in the equivalent genes in E. coli. This provides evidence that the genetic manipulation of lpx genes to generate altered lipid A molecules may be possible.
利用基于杂交的策略，已从b型流感嗜血杆菌中克隆出与大肠杆菌lpx基因（编码脂多糖A生物合成所需的酶）相似的基因。推导的氨基酸序列与其大肠杆菌对应序列高度同源。这些基因在大肠杆菌和流感嗜血杆菌中的排列顺序相同，但基因间区域不同。流感嗜血杆菌的lpxA和lpxB已在大肠杆菌小细胞中表达，且它们编码预测大小的蛋白质。流感嗜血杆菌的lpxA和lpxB都能够互补大肠杆菌中相应基因的温度敏感突变体。这为通过对lpx基因进行遗传操作来产生改变的脂多糖A分子提供了证据。
