Bilecik Şeyh Edebali University, Bioengineering Department, Bilecik, Turkiye.
Kutahya Dumlupinar University, Chemistry Department, Kutahya, Turkiye.
Toxicon. 2020 Nov;187:255-261. doi: 10.1016/j.toxicon.2020.09.005. Epub 2020 Sep 16.
In this study, we proposed label-free saxitoxin (STX) sensor using STX specific aptamer in combination with spectroscopic ellipsometry (SE) and attenuated internal reflection (AIR) spectroscopic ellipsometry method which is operated under surface plasmon resonance (SPR) conditions. Besides the other surface plasmon resonance-based applications, AIR-SE applications have unique advantages in terms of sensitivity and it was used herein for real-time detection of STX in real samples. Another method, SE, was also used and compared with AIR-SE. Analytical performances were satisfactory with low detection limits and a wide detection range. Limit of detection was 0.01 ng/mL for AIR-SE and 0.11 ng/mL for SE. Both proposed sensors were operable in 0.01 nM-1000 nM STX range. These methods were also used for the accurate, selective, and sensitive detection of STX from fish and shrimp samples.
在这项研究中，我们提出了一种无标记的石房蛤毒素（STX）传感器，该传感器结合了石房蛤毒素特异性适体，使用光谱椭圆偏振术（SE）和衰减全内反射（AIR）光谱椭圆偏振术方法，该方法在表面等离子体共振（SPR）条件下运行。除了其他基于表面等离子体共振的应用外，AIR-SE 应用在灵敏度方面具有独特的优势，本文将其用于实时检测实际样品中的 STX。还使用了另一种方法 SE，并与 AIR-SE 进行了比较。分析性能令人满意，具有较低的检测限和较宽的检测范围。AIR-SE 的检测限为 0.01ng/mL，SE 的检测限为 0.11ng/mL。两种传感器都可在 0.01 nM-1000 nM STX 范围内运行。这些方法还用于从鱼类和虾类样本中准确、选择性和灵敏地检测 STX。
