College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Toxins (Basel). 2024 Aug 2;16(8):341. doi: 10.3390/toxins16080341.
Saxitoxin (STX), an exceptionally potent marine toxin for which no antidote is currently available, is produced by methanogens and cyanobacteria. This poses a significant threat to both shellfish aquaculture and human health. Consequently, the development of a rapid, highly sensitive STX detection method is of great significance. The objective of this research is to create a novel approach for identifying STX. Therefore, amplified luminescent proximity homogeneous assay (AlphaLISA) was established using a direct competition method based on the principles of fluorescence resonance energy transfer and antigen-antibody specific binding. This method is sensitive, rapid, performed without washing, easy to operate, and can detect 8-128 ng/mL of STX in only 10 min. The limit of detection achieved by this method is as low as 4.29 ng/mL with coefficients of variation for the intra-batch and inter-batch analyses ranging from 2.61% to 3.63% and from 7.67% to 8.30%, respectively. In conclusion, our study successfully establishes a simple yet sensitive, rapid, and accurate AlphaLISA method for the detection of STX which holds great potential in advancing research on marine biotoxins.
石房蛤毒素（STX）是一种效力极强的海洋毒素，目前尚无解毒剂，由产甲烷菌和蓝藻产生。这对贝类养殖和人类健康构成了重大威胁。因此，开发一种快速、高灵敏度的 STX 检测方法具有重要意义。本研究旨在探索一种新的 STX 识别方法。因此，采用基于荧光共振能量转移和抗原抗体特异性结合原理的直接竞争法，建立了扩增发光邻近均相分析（AlphaLISA）。该方法灵敏、快速、无需洗涤、操作简便，仅需 10 分钟即可检测到 8-128ng/mL 的 STX。该方法的检测限低至 4.29ng/mL，批内和批间分析的变异系数分别为 2.61%-3.63%和 7.67%-8.30%。综上所述，本研究成功建立了一种简单、灵敏、快速、准确的 AlphaLISA 方法，用于检测 STX，为海洋生物毒素的研究提供了新的方法。
