Department of Analytical Chemistry, University of Granada, Faculty of Science, Av. Fuente Nueva, s/n, 18071 Granada, Spain; Unit of Excellence in Chemistry applied to Biomedicine and the Environment of the University of Granada, Spain.
Department of Inorganic Chemistry, University of Granada, Faculty of Science, Av. Fuente Nueva, s/n, 18071 Granada, Spain; Unit of Excellence in Chemistry applied to Biomedicine and the Environment of the University of Granada, Spain.
Colloids Surf B Biointerfaces. 2020 Dec;196:111337. doi: 10.1016/j.colsurfb.2020.111337. Epub 2020 Aug 27.
The determination of creatinine levels is essential for the detection of renal and muscular dysfunction. Luminescent nanoparticles are emerging as fast, cheap and highly selective sensors for the detection and quantification of creatinine. Nevertheless, current nanosensors only have a short shelf life due to their poor chemical and colloidal stability, which limits their clinical functionality. In this work, we have developed a highly stable, selective and sensitive nanosensor based on europium-doped, amorphous calcium phosphate nanoparticles (Eu-ACP) for the determination of creatinine by luminescence spectroscopy. The colloidal stability of Eu-ACP nanoparticles in aqueous solutions was optimised to ensure a constant signal after up to 4 months in storage. The luminescence intensity of Eu-ACP decreased linearly with the creatinine concentration over the range of 1-120 μM (R = 0.995). This concentration-response relationship was used to determine creatinine levels in real urine samples resulting in good recovery percentages. Significantly, selectivity assays indicated that none of the potential interfering species provoked discernible changes in the luminescence intensity.
肌酐水平的测定对于检测肾脏和肌肉功能障碍至关重要。发光纳米粒子作为快速、廉价和高选择性的肌酐检测和定量传感器正在兴起。然而，由于其化学和胶体稳定性差，目前的纳米传感器的保质期很短，这限制了它们的临床功能。在这项工作中，我们开发了一种基于掺铕的无定形磷酸钙纳米粒子（Eu-ACP）的高稳定性、选择性和灵敏的纳米传感器，通过荧光光谱法测定肌酐。优化了 Eu-ACP 纳米粒子在水溶液中的胶体稳定性，以确保在储存 4 个月后信号保持不变。Eu-ACP 纳米粒子的荧光强度随肌酐浓度在 1-120 μM 范围内呈线性降低（R = 0.995）。该浓度-响应关系用于测定实际尿样中的肌酐水平，回收率良好。值得注意的是，选择性测定表明，没有任何潜在的干扰物质引起荧光强度的明显变化。
