College of Horticulture and Plant Protection, Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
College of Horticulture and Plant Protection, Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Food Microbiol. 2020 Dec;92:103558. doi: 10.1016/j.fm.2020.103558. Epub 2020 Jun 6.
Brown rot caused by Monilinia fructicola has led to considerable preharvest and postharvest losses in all major nectarine fruit-growing areas. In our previous study, we successfully identified a biocontrol strain of bacteria, Bacillus licheniformis W10, that can be used to control brown rot. However, the possible mechanism of the control of brown rot by B. licheniformis W10 is still unclear. Therefore, the objectives of this study were to determine whether B. licheniformis W10 induces resistance by activating defense-related enzymes including antioxidant enzymes in nectarine. Treatment of nectarine fruit with B. licheniformis W10 reduced both M. fructicola-induced oxidative damage and reactive oxygen species (ROS) production. Furthermore, application of B. licheniformis to nectarine fruit resulted in a significant increase in the activity of antioxidant and defense-related enzymes and increase in the expression of the corresponding genes. Overall, our results verified the proposed mechanism of B. licheniformis W10 in controlling M. fructicola via regulation of ROS levels and activation of antioxidant and defense-related enzymes.
桃褐腐病由密粘褶菌引起，在所有主要油桃种植区都导致了相当大的采前和产后损失。在我们之前的研究中，我们成功地鉴定出一种可以用于控制褐腐病的细菌生物防治菌株，地衣芽孢杆菌 W10。然而，地衣芽孢杆菌 W10 控制褐腐病的可能机制仍不清楚。因此，本研究的目的是确定地衣芽孢杆菌 W10 是否通过激活包括桃抗氧化酶在内的防御相关酶来诱导抗性。用地衣芽孢杆菌 W10 处理油桃果实可降低桃褐腐病菌诱导的氧化损伤和活性氧（ROS）的产生。此外，将地衣芽孢杆菌应用于油桃果实可显著增加抗氧化和防御相关酶的活性，并增加相应基因的表达。总的来说，我们的结果验证了地衣芽孢杆菌 W10 通过调节 ROS 水平和激活抗氧化和防御相关酶来控制密粘褶菌的提出机制。
