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[卡诺氏液固定对兔颈上神经节核酸和蛋白质浓度的影响]

[Effect of fixation in Carnoy's solution on nucleic acid and protein concentration in the rabbit superior cervical sympathetic ganglion].

作者信息

Gorelikov P L

出版信息

Tsitologiia. 1977;19(1):90-4.

PMID:329504
Abstract

By means of biochemical techniques, the stability of RNA, DNA and protein contents has been demonstrated in the rabbit superior cervical sympathetic node fixed in Carnoy's solution for 2 hours at 4 degrees C. A 2 hour fixation in Carnoy's fluid at room temperature, results, however, in a considerable loss of RNA (21%). The loss of DNA and protein did not exceed 2% of the total amount of the fresh ganglion tissue. Fixation at a higher temperature (37%) increased the loss percentage protein and of nucleic acids: 3.1, 5.5 and 42%, for protein, DNA and RNA, resp.

摘要

通过生化技术,已证明在4℃下用卡诺氏液固定2小时的兔颈上神经节中,RNA、DNA和蛋白质含量具有稳定性。然而,在室温下用卡诺氏液固定2小时会导致RNA大量损失(21%)。DNA和蛋白质的损失不超过新鲜神经节组织总量的2%。在较高温度(37℃)下固定会增加蛋白质和核酸的损失百分比:蛋白质、DNA和RNA的损失百分比分别为3.1%、5.5%和42%。

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