Carreras I, Carreras B, McGrath L, Rice A, Easty D L
Departamento de Cirugía y sus Especialidades, Hospital Universitario, Granada, Spain.
Br J Ophthalmol. 1993 Aug;77(8):509-14. doi: 10.1136/bjo.77.8.509.
The aim of this study has been to determine whether the presence of lymphocytic infiltrates observed in the histology of ocular allergic conditions in humans or in the late phase of ocular anaphylactic reactions in experimental animals is a non-specific event dependent only on the degranulation of mast cells, or is conditioned by a specific response to antigen. With this in mind, responses to antigen and to a non-immunological mast cell degranulator (compound 48/80) were compared in an experimental model of allergic conjunctivitis. Rats were sensitised to ovalbumin and challenged topically in the left conjunctival sac either with ovalbumin or compound 48/80. The presence of T cells and activated T cells in the infiltrate was studied by immunohistochemical staining on conjunctival tissue obtained at 4, 24, and 48 hours after challenge. Ovalbumin sensitised and challenged rats showed increased numbers of T cells in the conjunctival infiltrate, statistically significant when compared with compound 48/80 challenged rats at 48 hours and with controls at 4, 24, and 48 hours. The number of T cells was significantly higher in compound 48/80 challenged rats only at 48 hours when compared with controls. As for the number of activated T cells, only ovalbumin sensitised and challenged rats showed significantly increased levels of these cells compared with both sensitised animals challenged with compound 48/80 and controls at 4 and 24 hours after challenge. These results suggest that the infiltration of the conjunctiva by activated T lymphocytes is, at least in part, dependent on a specific response to antigen.
本研究的目的是确定在人类眼部过敏性疾病组织学检查或实验动物眼部过敏反应后期观察到的淋巴细胞浸润是仅依赖于肥大细胞脱颗粒的非特异性事件，还是由对抗原的特异性反应所决定。考虑到这一点，在过敏性结膜炎实验模型中比较了对抗原和非免疫性肥大细胞脱颗粒剂（化合物48/80）的反应。用卵清蛋白使大鼠致敏，然后在左结膜囊局部用卵清蛋白或化合物48/80进行激发。在激发后4、24和48小时获得的结膜组织上进行免疫组织化学染色，研究浸润物中T细胞和活化T细胞的存在情况。卵清蛋白致敏并激发的大鼠结膜浸润物中的T细胞数量增加，与化合物48/80激发的大鼠在48小时时以及与对照组在4、24和48小时时相比，具有统计学意义。仅在48小时时，化合物48/80激发的大鼠中的T细胞数量与对照组相比显著更高。至于活化T细胞的数量，与用化合物48/80激发的致敏动物以及激发后4和24小时的对照组相比，仅卵清蛋白致敏并激发的大鼠显示这些细胞水平显著增加。这些结果表明，活化T淋巴细胞对结膜的浸润至少部分依赖于对抗原的特异性反应。
