Clinical Medical Research Center, and Department of Oncology, Inner Mongolia People's Hospital, No. 20 Zhaowuda Road, Saihan District, Hohhot 010017, PR China.
Department of Oncology, Inner Mongolia People's Hospital, Hohhot 010017, PR China.
Dig Liver Dis. 2021 Feb;53(2):238-244. doi: 10.1016/j.dld.2020.08.045. Epub 2020 Sep 18.
Increasing evidence showed that Heart and Neural Crest Derivatives Expressed 2 antisense RNA 1 (HAND2-AS1) was involved in the progression of several cancers, but its expression and function in gastric cancer (GC) was rarely reported. HAND2-AS1 expression in GC tissues and cells was detected at first. Cell function assays were performed to investigate the biological roles of HAND2-AS1 in GC cells. Moreover, the genes regulated by HAND2-AS1 in GC were investigated. Downregulation of HAND2-AS1 was found in GC tissues and cell lines. HAND2-AS1 overexpression inhibited GC cell proliferation, invasion, and arrested cell cycle at G0/G1 phase, whereas HADN2-AS1 knockdown significantly promoted cell proliferation and invasion. Bioinformatic analysis showed there is a potential HADN2-AS1/microRNA-769-5p (miR-769-5p)/transcription elongation factor A like 7 (TCEAL7) axis in GC. Luciferase activity reporter system was used to confirm this link. Taken together, our study showed that HAND2-AS1 exerts its tumor suppressive role in GC via regulating miR-769-5p/TCEAL7.
越来越多的证据表明，心和神经嵴衍生物表达 2 反义 RNA1（HAND2-AS1）参与了几种癌症的进展，但它在胃癌（GC）中的表达和功能很少有报道。首先检测了 GC 组织和细胞中 HAND2-AS1 的表达。进行细胞功能测定以研究 HAND2-AS1 在 GC 细胞中的生物学作用。此外，还研究了 HAND2-AS1 在 GC 中调节的基因。GC 组织和细胞系中发现 HAND2-AS1 下调。HAND2-AS1 的过表达抑制 GC 细胞增殖、侵袭，并将细胞周期阻滞在 G0/G1 期，而 HADN2-AS1 的敲低则显著促进细胞增殖和侵袭。生物信息学分析表明，GC 中存在 HAND2-AS1/微小 RNA-769-5p（miR-769-5p）/转录延伸因子 A 样 7（TCEAL7）轴。荧光素酶活性报告系统用于证实这一联系。总之，我们的研究表明 HAND2-AS1 通过调节 miR-769-5p/TCEAL7 在 GC 中发挥其肿瘤抑制作用。
