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Utility of a modified heat inactivation method for direct detection of SARS-CoV-2 by RT-qPCR in viral transport medium bypassing RNA extraction: A preliminary study.

作者信息

Alagarasu Kalichami, Potdar Varsha A, Vipat Veena, Hundekar Supriya, Gunjikar Rashmi, Choudhary Manohar L, Abraham Priya, Lole Kavita S

机构信息

Dengue/Chikungunya Group, ICMR-National Institute of Virology, Pune 411 001, Maharashtra, India.

Human Influenza Group, ICMR-National Institute of Virology, Pune 411 001, Maharashtra, India.

出版信息

Indian J Med Res. 2020;152(1 & 2):108-110. doi: 10.4103/ijmr.IJMR_3121_20.

DOI:10.4103/ijmr.IJMR_3121_20
PMID:32952145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7853279/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b7/7853279/04add463482a/IJMR-152-108-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b7/7853279/04add463482a/IJMR-152-108-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c5b7/7853279/04add463482a/IJMR-152-108-g001.jpg

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J Clin Virol. 2020 Jul;128:104423. doi: 10.1016/j.jcv.2020.104423. Epub 2020 May 7.
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Overcoming the bottleneck to widespread testing: a rapid review of nucleic acid testing approaches for COVID-19 detection.克服广泛检测的瓶颈：对 COVID-19 检测的核酸检测方法的快速综述。
RNA. 2020 Jul;26(7):771-783. doi: 10.1261/rna.076232.120. Epub 2020 May 1.
3
COVID-19: fail to prepare, prepare to fail.新型冠状病毒肺炎：不做准备，就准备失败。
J R Soc Med. 2020 Apr;113(4):131. doi: 10.1177/0141076820918796.
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Development of transcribed RNA as positive control for laboratory diagnosis of SARS-CoV-2 in India.转录RNA作为印度SARS-CoV-2实验室诊断阳性对照的开发。
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An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing.一种优化的从激光捕获微切割 FFPE 组织小切片中分离 RNA 的方案，适用于下一代测序。
BMC Mol Biol. 2017 Aug 23;18(1):22. doi: 10.1186/s12867-017-0099-7.
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Endogenous RNase inhibitor contributes to stability of RNA in crude cell lysates: Applicability to RT-qPCR.内源性核糖核酸酶抑制剂有助于粗细胞裂解物中RNA的稳定性：在逆转录-定量聚合酶链反应中的适用性。
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