SARS-CoV-2 检测的实验室管理：热处理方法与 rt-Real-time PCR 检测的便捷组合。
Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing.
机构信息
Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.
European Public Health Microbiology Training Program (EUPHEM), European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden.
出版信息
Emerg Microbes Infect. 2020 Dec;9(1):1393-1396. doi: 10.1080/22221751.2020.1775500.
Abstract
The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.
摘要
RNA 纯化是检测拭子样本中 SARS-CoV-2 的金标准，但它依赖于化学试剂的可用性。在本研究中，我们评估了无需 RNA 提取的热处理方法作为一种可靠的核酸纯化选择。
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