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An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020.SARS-CoV-2 分子检测的替代工作流程-摆脱核酸提取试剂盒短缺，丹麦哥本哈根，2020 年 3 月。

Euro Surveill. 2020 Apr;25(14). doi: 10.2807/1560-7917.ES.2020.25.14.2000398.

2

Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay Kit.七种不同引物探针组合和一种检测试剂盒检测 SARS-CoV-2 的比较性能。

J Clin Microbiol. 2020 May 26;58(6). doi: 10.1128/JCM.00557-20.

3

Whole genome and phylogenetic analysis of two SARS-CoV-2 strains isolated in Italy in January and February 2020: additional clues on multiple introductions and further circulation in Europe.2020 年 1 月和 2 月在意大利分离的两株 SARS-CoV-2 病毒的全基因组和系统进化分析：关于多次传入和在欧洲进一步传播的更多线索。

Euro Surveill. 2020 Apr;25(13). doi: 10.2807/1560-7917.ES.2020.25.13.2000305.

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Detection of SARS-CoV-2 in Different Types of Clinical Specimens.SARS-CoV-2 在不同类型临床标本中的检测。

JAMA. 2020 May 12;323(18):1843-1844. doi: 10.1001/jama.2020.3786.

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The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2.严重急性呼吸综合征相关冠状病毒：将 2019-nCoV 进行分类并命名为 SARS-CoV-2。

Nat Microbiol. 2020 Apr;5(4):536-544. doi: 10.1038/s41564-020-0695-z. Epub 2020 Mar 2.

6

Combination of RT-qPCR testing and clinical features for diagnosis of COVID-19 facilitates management of SARS-CoV-2 outbreak.采用逆转录定量聚合酶链反应（RT-qPCR）检测与临床特征相结合的方法诊断新型冠状病毒肺炎（COVID-19），有助于对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）疫情进行管控。

J Med Virol. 2020 Jun;92(6):538-539. doi: 10.1002/jmv.25721. Epub 2020 Mar 11.

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SARS-CoV-2 Viral Load in Upper Respiratory Specimens of Infected Patients.感染患者上呼吸道标本中的新型冠状病毒2型病毒载量

N Engl J Med. 2020 Mar 19;382(12):1177-1179. doi: 10.1056/NEJMc2001737. Epub 2020 Feb 19.

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Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.实时 RT-PCR 检测 2019 新型冠状病毒（2019-nCoV）

Euro Surveill. 2020 Jan;25(3). doi: 10.2807/1560-7917.ES.2020.25.3.2000045.

9

A novel coronavirus outbreak of global health concern.一场引发全球卫生关注的新型冠状病毒疫情。

Lancet. 2020 Feb 15;395(10223):470-473. doi: 10.1016/S0140-6736(20)30185-9. Epub 2020 Jan 24.

SARS-CoV-2 检测的实验室管理：热处理方法与 rt-Real-time PCR 检测的便捷组合。

Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing.

机构信息

Department of Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy.

European Public Health Microbiology Training Program (EUPHEM), European Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden.

出版信息

Emerg Microbes Infect. 2020 Dec;9(1):1393-1396. doi: 10.1080/22221751.2020.1775500.

DOI:10.1080/22221751.2020.1775500

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7473159/

Abstract

The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.

摘要

RNA 纯化是检测拭子样本中 SARS-CoV-2 的金标准，但它依赖于化学试剂的可用性。在本研究中，我们评估了无需 RNA 提取的热处理方法作为一种可靠的核酸纯化选择。