Das M K, Subramanyam V R, Ravindran B, Pattnaik N M
J Trop Med Hyg. 1987 Jun;90(3):135-41.
Circulating antigens, antibodies to somatic and sheath components of microfilariae (mf) and immune complexes were determined in parallel in different categories of Wuchereia bancrofti infection using, respectively, reverse indirect haemagglutination (RIHA), indirect haemagglutination (IHA), indirect immunofluorescent (IFA) and polyethylene glycol (PEG) precipitation assays. Rabbit hyperimmune anti-W. bancrofti mf serum and mf homogenates were used as reagents. Appreciable levels of antigens and antibodies were detected in all categories; endemic normals, asymptomatic carriers, acute and chronic filarial cases. For example, even amongst endemic normals, i.e. those with neither clinical nor parasitological evidence of infection, 66%, 71% and 74% had mf antigens, anti-mf antibodies and anti-sheath antibodies, respectively. Notably, only a small proportion (9.4%) of microfilaraemic individuals had detectable level of anti-sheath antibodies. The relationship of these parameters with the spectrum of filariasis is discussed.
采用反向间接血凝试验（RIHA）、间接血凝试验（IHA）、间接免疫荧光试验（IFA）和聚乙二醇（PEG）沉淀试验，分别对不同类型的班氏吴策线虫感染患者的循环抗原、抗微丝蚴（mf）体壁和鞘膜成分抗体以及免疫复合物进行了平行检测。使用兔超免疫抗班氏吴策线虫mf血清和mf匀浆作为试剂。在所有类型中均检测到了可观水平的抗原和抗体，包括地方性正常人群、无症状携带者、急性和慢性丝虫病患者。例如，即使在地方性正常人群中，即那些既无感染临床证据也无寄生虫学证据的人群中，分别有66%、71%和74%的人存在mf抗原、抗mf抗体和抗鞘膜抗体。值得注意的是，仅有一小部分（9.4%）微丝蚴血症患者的抗鞘膜抗体水平可检测到。本文讨论了这些参数与丝虫病谱的关系。
