Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Lower Silesia, Poland.
Division of Colorectal Surgery, Department of Surgery, University of California Irvine (UCI), Orange, CA, USA.
Int J Surg Oncol. 2020 Aug 25;2020:9679385. doi: 10.1155/2020/9679385. eCollection 2020.
Micro- and nanoparticles, with their submicron size, the versatility of physical and chemical properties, and easily modifiable surface, are uniquely positioned to bypass the body's clearing systems. Nonetheless, two main problems with micro- and nanoparticles arise which limit the intraperitoneal application. The study was performed to evaluate whether HIUS enables the imprinting of microparticles and, therefore, enhances penetration and local endurance in the peritoneum.
High-intensity ultrasound (HIUS) at 20 kilohertz with an output power of 70 W was applied on peritoneal tissue samples from fresh postmortem swine for different time intervals. Before the HIUS application, the surface of the samples was covered with strontium aluminate microparticles before analysis via electron microscopy. In-tissue strontium aluminate penetration and particle distribution size were measured using fluorescence microscopy on frozen thin sections.
With increasing HIUS durations (1 versus 5 minutes), increasing strontium aluminate particles were detected in the peritoneum. HIUS leads to a particle selection process with enhancing predominantly the penetration of smaller particles whereas larger particles had a harder time penetrating the peritoneum. Smaller particles were detected up to 277 m ± 86 m into the peritoneum.
Our data indicate that HIUS might be used as a method to prepare the peritoneal tissue for micro- and nanoparticles. Higher tissue penetration rates without the increase and longer local endurance of the applied substance could be reached. More studies need to be performed to analyze the effect of HIUS in enhancing intraperitoneal drug applications.
由于具有亚微米级的尺寸、多样化的物理和化学性质以及易于修饰的表面，微纳米颗粒能够绕过人体的清除系统。然而，微纳米颗粒存在两个主要问题，限制了其在腹腔内的应用。本研究旨在评估高强度超声（HIUS）是否能够对微颗粒进行印迹，从而增强其在腹膜中的穿透性和局部持久性。
采用 20 千赫兹、70 瓦输出功率的高强度超声（HIUS）对新鲜猪死后的腹膜组织样本进行不同时间间隔的处理。在 HIUS 应用之前，在样本表面覆盖锶铝酸盐微颗粒，然后通过电子显微镜进行分析。在冷冻的薄片上使用荧光显微镜测量组织内锶铝酸盐的穿透和颗粒分布大小。
随着 HIUS 持续时间（1 分钟与 5 分钟）的增加，腹膜内检测到的锶铝酸盐颗粒数量增加。HIUS 导致了一个颗粒选择过程，主要增强了较小颗粒的穿透性，而较大颗粒则更难穿透腹膜。较小的颗粒被检测到穿透到腹膜内 277 ± 86 µm 的深度。
我们的数据表明，HIUS 可能被用作一种预处理腹膜组织的方法，用于微纳米颗粒。可以达到更高的组织穿透率，而不会增加应用物质的量和延长其局部持续时间。需要进一步研究来分析 HIUS 在增强腹腔内药物应用方面的效果。
