Karimi Sareh, Tabatabaei Seyed Nasrollah, Gutleb Arno C, Ghaffari Novin Marefat, Ebrahimzadeh-Bideskan Alireza, Shams Mofarahe Zahra
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Heliyon. 2020 Sep 6;6(9):e04862. doi: 10.1016/j.heliyon.2020.e04862. eCollection 2020 Sep.
Today, nanotechnology plays an important role in our ever-continuous quest to improve the quality of human life. Because of their infinitesimal size, nanostructures can actively interact and alter cellular functions. Therefore, while the clinical benefits of nanotechnology may outweigh most of the associated risks, assessment of the cytotoxicity of nanostructures in respect to cells and tissues early in product development processes is of great significance. To the best of our knowledge, no such assessment has been performed for nanomaterials on the ovarian cortex before. Herein, silica-coated, PEGylated silica-coated, and uncoated iron oxide nanoparticles (IONP) with core diameter of 11 nm (±4.2 nm) were synthesized. The oxidative stress in cultured ovarian tissue exposed to the various IONP was subsequently assessed. The results indicate that among the four groups, uncoated IONP induce the most oxidative stress on the ovarian cortex while tissues treated with PEGylated IONP exhibit no significant change in oxidative stress.
如今，纳米技术在我们不断追求改善人类生活质量的过程中发挥着重要作用。由于其极小的尺寸，纳米结构能够积极地与细胞相互作用并改变细胞功能。因此，虽然纳米技术的临床益处可能超过大多数相关风险，但在产品开发过程早期评估纳米结构对细胞和组织的细胞毒性具有重要意义。据我们所知，此前尚未对纳米材料在卵巢皮质上进行过此类评估。在此，合成了核心直径为11纳米（±4.2纳米）的二氧化硅包覆、聚乙二醇化二氧化硅包覆和未包覆的氧化铁纳米颗粒（IONP）。随后评估了暴露于各种IONP的培养卵巢组织中的氧化应激。结果表明，在四组中，未包覆的IONP对卵巢皮质诱导的氧化应激最大，而用聚乙二醇化IONP处理的组织在氧化应激方面没有显著变化。
