Advance Science and Technology Research Centre, Vinoba Bhave University, Hazaribagh, Jharkhand, 825301, India; University Department of Biotechnology, Vinoba Bhave University, Hazaribag, 825301, India.
Advance Science and Technology Research Centre, Vinoba Bhave University, Hazaribagh, Jharkhand, 825301, India.
Environ Pollut. 2020 Dec;267:115482. doi: 10.1016/j.envpol.2020.115482. Epub 2020 Aug 25.
Extensive use of magnetic iron oxide (magnetite) nanoparticles (IONP) has raised concerns about their biocompatibility. It has also stimulated the search for its green synthesis with greater biocompatibility. Addressing the issue, this study investigates the molecular nanotoxicity of IONP with embryonic and adult zebrafish, and reveal novel green fabrication of iron oxide nanoparticles (P-IONP) using medicinal plant extract of Phyllanthus niruri. The synthesized P-IONP was having a size of 42 ± 08 nm and a zeta potential of -38 ± 06 mV with hydrodynamic diameter of 109 ± 09 nm and 90emu/g magnetic saturation value. High antibacterial efficacy of P-IONP was found against E.coli. Comparative in vivo biocompatibility assessment with zebrafish confirmed higher biocompatibility of P-IONP compared to commercial C-IONP in the relevance of mortality rate, hatching rate, heart rate, and morphological abnormalities. LC of P-IONP and C-IONP was 202 μg/ml and 126 μg/ml, respectively. Molecular nano-biocompatibility analysis revealed the phenomenon as an effect of induced apoptosis lead by dysregulation of induced oxidative stress due to structural and functional influence of IONP to Sod1 and Tp53 proteins through intrinsic atomic interaction.
磁性氧化铁（磁铁矿）纳米粒子（IONP）的广泛应用引起了人们对其生物相容性的关注。这也刺激了人们寻找具有更高生物相容性的绿色合成方法。针对这一问题，本研究利用胚胎和成年斑马鱼研究了 IONP 的分子纳米毒性，并揭示了一种使用药用植物叶下珠提取物制造氧化铁纳米粒子（P-IONP）的新的绿色方法。合成的 P-IONP 粒径为 42 ± 08nm，zeta 电位为-38 ± 06mV，水动力直径为 109 ± 09nm，饱和磁化强度为 90emu/g。研究发现，P-IONP 对大肠杆菌具有很强的抗菌效果。与斑马鱼进行的体内比较生物相容性评估证实，与商用 C-IONP 相比，P-IONP 的生物相容性更高，表现在死亡率、孵化率、心率和形态异常方面。P-IONP 和 C-IONP 的 LC 分别为 202μg/ml 和 126μg/ml。分子纳米生物相容性分析表明，这种现象是由于 IONP 对 Sod1 和 Tp53 蛋白的结构和功能的影响，导致诱导氧化应激的失调，从而引发细胞凋亡。
