Industrial Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea.
College of Pharmacy, Chungnam National University, Daejeon, South Korea.
Pest Manag Sci. 2021 Apr;77(4):1607-1615. doi: 10.1002/ps.6095. Epub 2021 Jan 15.
Pine wilt disease (PWD) is a plant disease that causes serious damage to pine trees. PWD occurs when the host plant is infected with pinewood nematode (PWN), Bursaphelenchus xylophilus. In this study, a compound with nematicidal activity was isolated from actinomycetes and its efficacy was investigated in vitro.
We screened and selected Streptomyces sp. 680560, which had nematicidal activity against B. xylophilus. Based on 16S rRNA sequence analysis, it showed 99.93% similarity with Streptomyces blastmyceticus NRRB-5480 . Furthermore, the active compound was isolated and identified as teleocidin B4. Teleocidin B4 at concentrations ranging from 6.25 to 100 μM had low nematicidal activity after 24 and 36 h against adult and stage juveniles (J2) of B. xylophilus, but after 48 h nematicidal activity exceeded 95%. The rate of inhibition of egg hatching for Teleocidin B4 6.25, 12.5, 25, 50, and 100 μM was confirmed to be dose-dependently inhibited after 48 h of treatment. Teleocidin B4 is not only toxic to hatched B. xylophilus, but also affects egg hatching.
This study was carried out to isolate actinomycete metabolites from pine tree endophytes from various natural environments for control of PWD. A compound with nematicidal activity was isolated from a selected strain and its structure was identified as teleocidin B4. The nematicidal effect of the isolated active substance, teleocidin B4, was confirmed. This is the first report of the effect of teleocidin B4 on B. xylophilus, suggesting its possibility as a PWD control agent. © 2020 Society of Chemical Industry.
松材线虫病（PWD）是一种对松树造成严重损害的植物病害。当宿主植物感染松材线虫（Bursaphelenchus xylophilus）时，就会发生 PWD。在这项研究中，从放线菌中分离出一种具有杀线虫活性的化合物，并在体外研究了其功效。
我们筛选并选择了对 B. xylophilus 具有杀线虫活性的链霉菌 sp. 680560。基于 16S rRNA 序列分析，它与 Streptomyces blastmyceticus NRRB-5480 表现出 99.93%的相似性。此外，分离并鉴定出活性化合物为 teleocidin B4。在 24 和 36 小时时，浓度在 6.25 至 100 μM 之间的 teleocidin B4 对 B. xylophilus 的成虫和第 2 期幼虫（J2）的杀线虫活性较低，但 48 小时后，杀线虫活性超过 95%。在 48 小时的处理后，证实 teleocidin B4 6.25、12.5、25、50 和 100 μM 对卵孵化的抑制率呈剂量依赖性抑制。
本研究从各种自然环境的松树内生菌中分离放线菌代谢产物，用于控制 PWD。从选定的菌株中分离出具有杀线虫活性的化合物，并鉴定其结构为 teleocidin B4。分离的活性物质 teleocidin B4 的杀线虫效果得到了证实。这是 teleocidin B4 对 B. xylophilus 影响的首次报道，表明其作为 PWD 控制剂的可能性。© 2020 化学工业协会。
