Department of Microbiology, Vall d'Hebron University Hospital, Universitat Autònoma de Barcelona, PROSICS Barcelona, Spain.
Departamento de Clínica Médica, Unidade de Cardiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP-USP), Brazil.
Future Microbiol. 2020 Aug;15:1139-1145. doi: 10.2217/fmb-2020-0023.
This work aimed to compare the sensitivity of four protocols for the detection of DNA in 98 blood samples from chronic Chagas disease patients. Two DNA extraction (automated and manual) methods and two satellite DNA qPCRs (with a recent design and the usually used set of primers) were analyzed. Both DNA extraction methods and qPCR assays tested in this work gave comparable qualitative results, although the lowest Ct values were obtained when samples were analyzed using the new set of primers for satellite DNA. Our results encourage the implementation of automated DNA extraction systems and the new qPCR for the molecular diagnostics and treatment response monitoring of chronic Chagas disease patients.
本研究旨在比较四种方案检测 98 份慢性恰加斯病患者血液样本中 DNA 的灵敏度。分析了两种 DNA 提取方法（自动和手动）和两种卫星 DNA qPCR（采用最新设计和常用引物集）。本研究中测试的两种 DNA 提取方法和 qPCR 检测方法均给出了可比的定性结果，尽管使用新的卫星 DNA 引物组分析样本时获得的 Ct 值最低。我们的结果鼓励采用自动化 DNA 提取系统和新的 qPCR 进行慢性恰加斯病患者的分子诊断和治疗反应监测。
