表型检测群体感应抑制物在绿脓菌素和挥发性有机产物的群集运动中的作用。

Phenotypic detection of quorum sensing inhibition in pyoverdine and swarming by volatile organic products.

机构信息

Department of Plant Biology, PPG BTPB & PPG BV, Institute of Biology, University of Campinas - UNICAMP, Postal Box 6109, Campinas - SP 13083-970, Brazil.

Faculty of Health Sciences. Pedagogical & Technological University of Colombia, Postal Box 0387437173 Tunja, Calle 24 N° 5-63, Colombia.

出版信息

Future Microbiol. 2020 Aug;15:1147-1156. doi: 10.2217/fmb-2020-0033.

DOI:10.2217/fmb-2020-0033

PMID:32954846

Abstract

To determine phenotypically the anti quorum-sensing (QS) activity of 30 volatile organic products (VOPs) through the inhibition of swarming motility and pyoverdine production in . Twenty-four essential oils and six small volatile organic compounds randomly selected were screened for their anti-QS activity by violacein inhibition on . The VOPs with positive results were subsequently evaluated for swarming motility and pyoverdine production on determining the colony diameter and fluorescence under UV light, respectively. Fifty percent of VOPs tested showed strong violacein inhibition, 40% presented anti-swarming activity and 33% inhibited pyoverdine production. Our data demonstrate that VOPs have a great potential to inhibit virulence factors mediated by QS in .

摘要

通过抑制 swarm 运动和绿脓菌素产生来表型确定 30 种挥发性有机产物 (VOPs) 的抗群体感应 (QS) 活性。随机选择了 24 种精油和 6 种小分子挥发性有机化合物，通过对. 的紫霉素抑制来筛选其抗 QS 活性。具有阳性结果的 VOPs 随后在. 上进行 swarm 运动和绿脓菌素产生的评估，分别通过测定菌落直径和在 UV 光下的荧光来确定。测试的 VOPs 中有 50%表现出强烈的紫霉素抑制作用，40%表现出抗 swarm 活性，33%抑制绿脓菌素产生。我们的数据表明，VOPs 具有很大的潜力来抑制. 中由 QS 介导的毒力因子。