Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot, China; Department of Pathology, the Affiliated Hospital of Inner Mongolia Medical University, Inner Mongolia Medical University, Hohhot, China.
Reproductive Medicine Center, the Affiliated Hospital of Inner Mongolia Medical University, Inner Mongolia Medical University, Hohhot, China.
Res Vet Sci. 2020 Dec;133:92-97. doi: 10.1016/j.rvsc.2020.09.002. Epub 2020 Sep 8.
Connexin 43 (Cx43), known to form gap junction transmembrane channels between the cytoplasm of two adjacent cells, plays a key role in physiological functions, such as regulating cell growth, differentiation, and maintaining tissue homeostasis. Cashmere goat is an important farm animal that provides cashmere, which was produced by secondary hair follicles (SHF), for human consumption; however, there is no report about the role of Cx43 on the growth and development of SHF in cashmere goat. In this study, we investigated the effect of Cx43 on proliferation secondary hair follicle dermal papilla cells (SHF-DPCs) in Albas cashmere goat. In SHF-DPCs, Cx43 overexpression promoted cell proliferation and upregulated the expression of IGF-1, whereas Cx43 knockdown was associated with the opposite effects. These results suggested that Cx43 may promote cell proliferation by inducing IGF-1. Overall, our research not only contributes to a better understanding of the mechanism of the growth and development of SHF in cashmere goat, but also shed light on cashmere quality control in the future.
间隙连接蛋白 43（Cx43），已知在两个相邻细胞的细胞质之间形成间隙连接跨膜通道，在生理功能中发挥关键作用，如调节细胞生长、分化和维持组织内稳态。绒山羊是一种重要的家畜，提供用于人类消费的羊绒，它是由次级毛囊（SHF）产生的；然而，关于 Cx43 在绒山羊 SHF 的生长和发育中的作用尚无报道。在这项研究中，我们研究了 Cx43 对阿尔巴斯绒山羊 SHF 真皮乳头细胞（SHF-DPC）增殖的影响。在 SHF-DPC 中，Cx43 的过表达促进细胞增殖并上调 IGF-1 的表达，而 Cx43 的敲低则与之相反。这些结果表明 Cx43 可能通过诱导 IGF-1 促进细胞增殖。总的来说，我们的研究不仅有助于更好地理解绒山羊 SHF 生长和发育的机制，而且为未来的羊绒质量控制提供了思路。
