Wolf J P, Dumoulin G, Henriet M T, Baulay A, Berthelay S
Pathol Biol (Paris). 1987 Apr;35(4):422-6.
Two methods of extraction, prior to radio-immunoassay (RIA) of human plasma arginine vasopressin (AVP) were tested: ethanol extraction (ETH), chromatography with ODS-Silica (ODS-Sil). Recovery of 125I AVP was higher when using ODS-Sil than when using ETH. Recovery of standard AVP and plasma enriched with standard AVP was found to be more efficient and reproducible for ODS-Sil. However, the RIA used, performed after chromatography with ODS-Sil, is not enough sensitive to detect low concentrations but is able to detect high concentrations and physiological variations of plasma AVP.
在对人血浆精氨酸加压素（AVP）进行放射免疫分析（RIA）之前，测试了两种提取方法：乙醇提取（ETH）、ODS-硅胶柱色谱法（ODS-Sil）。使用ODS-Sil时，125I-AVP的回收率高于使用ETH时。对于ODS-Sil，标准AVP和富含标准AVP的血浆的回收率更高且可重复。然而，在使用ODS-Sil柱色谱法之后进行的RIA，检测低浓度时灵敏度不足，但能够检测血浆AVP的高浓度及生理变化。
