Kalmár L, Láng I, Gonzelez-Cabello P, Gergely P, Fehér J
Acta Med Hung. 1986;43(3):255-63.
The effect of three non-steroidal anti-inflammatory agents (NSAIs) and of two new pyrido-pyrimidine derivatives, CHINOIN 127 (1-6-dimethyl-4-oxo-1,6,7,8,9)a-hexahydro-4H-pyrido (1,2-2)pyrimidine-3-carboxamide) and CHINOIN 105 (1-6-dimethyl-4-oxo-1,6,7,8-tetrahydro-4H pyrido (1,2-a) pyrimidine-3-carboxamide) was compared in immune tests using human cells in vitro including T and B lymphocyte proliferation induced with mitogens, spontaneous motility of polymorphonuclear leukocytes, lymphokine (LIF) production, antibody-dependent cell-mediated cytotoxicity (ADCC) and natural killer cell (NK) activity. Indomethacin (INDO) at therapeutic concentrations enhanced the proliferation of lymphocytes stimulated by both Con A and PWM. Acetylsalicylic acid (ASA) increased proliferation of lymphocytes stimulated by Con A and at high concentration inhibited DNA synthesis induced by PWM. Phenylbutazone (PhB) increased DNA synthesis induced by PWM but not by Con A. CHINOIN 127 and CHINOIN 105 inhibited the proliferation of T lymphocytes but failed to influence DNA synthesis of B cells. Indomethacin, ASA and PhB inhibited the spontaneous motility (migration) of polymorphonuclear leukocytes. LIF production of lymphocytes was inhibited by INDO, ASA, PhB, CHINOIN 127 and CHINOIN 105. Phenylbutazone and ASA inhibit ADCC and NK. INDO, Chinoin 127 and Chinoin 105 failed to influence the effector cells of ADC and NK cytotoxicity.
在体外用人细胞进行的免疫试验中，比较了三种非甾体抗炎药（NSAIs）以及两种新型吡啶并嘧啶衍生物（CHINOIN 127，即1-6-二甲基-4-氧代-1,6,7,8,9α-六氢-4H-吡啶并[1,2-α]嘧啶-3-甲酰胺；CHINOIN 105，即1-6-二甲基-4-氧代-1,6,7,8-四氢-4H-吡啶并[1,2-α]嘧啶-3-甲酰胺）的作用，这些试验包括有丝分裂原诱导的T和B淋巴细胞增殖、多形核白细胞的自发运动、淋巴因子（LIF）产生、抗体依赖性细胞介导的细胞毒性（ADCC）以及自然杀伤细胞（NK）活性。治疗浓度的吲哚美辛（INDO）增强了刀豆蛋白A（Con A）和美洲商陆有丝分裂原（PWM）刺激的淋巴细胞增殖。乙酰水杨酸（ASA）增加了Con A刺激的淋巴细胞增殖，且在高浓度时抑制PWM诱导的DNA合成。保泰松（PhB）增加了PWM诱导的DNA合成，但未增加Con A诱导的DNA合成。CHINOIN 127和CHINOIN 105抑制T淋巴细胞增殖，但未影响B细胞的DNA合成。吲哚美辛、ASA和PhB抑制多形核白细胞的自发运动（迁移）。INDO、ASA、PhB、CHINOIN 127和CHINOIN 105抑制淋巴细胞的LIF产生。保泰松和ASA抑制ADCC和NK。INDO、CHINOIN 127和CHINOIN 105未影响ADCC和NK细胞毒性的效应细胞。
