School of Environment, South China Normal University, University Town, Guangzhou, 510006, Guandong, China.
Department of Physics and Astronomy, College of Science, King Saud University, P.O. Box-2455, Riyadh, 11451, Kingdom of Saudi Arabia.
Bioprocess Biosyst Eng. 2021 Feb;44(2):355-368. doi: 10.1007/s00449-020-02447-w. Epub 2020 Sep 21.
This study aimed to characterize the biofilm microbial community that causes corrosion of API 5LX carbon steel. API 5LX carbon steel coupons were incubated with raw produced water collected from two oil reservoir stations or filter-sterilized produced water. Biofilm 16S rRNA amplicon sequencing revealed that the bacterial community present in the biofilm was dominated by Proteobacteria, including Marinobacter hydrocarbonoclaustics and Marinobacter alkaliphilus. Electrochemical analysis such as impedance and polarization results indicated that Proteobacteria biofilm accelerated corrosion by ~ twofold (2.1 ± 0.61 mm/years) or ~ fourfold (~ 3.7 ± 0.42 mm/years) when compared to the control treatment (0.95 ± 0.1 mm/years). Scanning electron and atomic force microscopy revealed the presence of a thick biofilm and pitting corrosion. X-ray diffraction revealed higher amounts of the corrosion products FeO, γ-FeOOH, and α-FeOOH, and confirmed that the microbial biofilm strongly oxidized the iron and contributed to the acceleration of corrosion of carbon metal API 5LX.
本研究旨在描述 API 5LX 碳钢腐蚀的生物膜微生物群落。从两个油藏站采集的原始采出水或过滤后的采出水孵育 API 5LX 碳钢试片，对生物膜 16S rRNA 扩增子测序表明，生物膜中的细菌群落主要由变形菌门（Proteobacteria）组成，包括海杆菌（Marinobacter hydrocarbonoclaustics）和海碱杆菌（Marinobacter alkaliphilus）。电化学分析（如阻抗和极化结果）表明，与对照处理（0.95±0.1mm/年）相比，当 Proteobacteria 生物膜存在时，腐蚀速度加快了约两倍（2.1±0.61mm/年）或约四倍（~3.7±0.42mm/年）。扫描电子显微镜和原子力显微镜揭示了厚生物膜和点蚀腐蚀的存在。X 射线衍射揭示了更多的腐蚀产物 FeO、γ-FeOOH 和 α-FeOOH，并证实微生物生物膜强烈氧化了铁，促进了 API 5LX 碳钢的腐蚀加速。
