College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
Int J Syst Evol Microbiol. 2020 Nov;70(11):5964-5968. doi: 10.1099/ijsem.0.004474.
In 2018, Nouioui transferred and to as subsp. and subsp. on the basis of digital DNA-DNA hybridization (dDDH) values. These two new subspecies were validated in the same year. However, we found that the genome (GenBank/ENA/DDBJ accession number JGZJ01000000) of used by Nouioui in the dDDH analysis cannot represent . So, the taxonomic relationship between , and should be re-examined. DSM 20433 had 88.7-89.0 % average nucleotide identity (ANI) values and 37.5-38.0 % dDDH values to the type strains of and , respectively, less than the threshold for species demarcation, confirming that represents a different species from and . The ANI values and dDDH values between the type strains of and were 96.7-96.9 % and 73.0-73.3 %, respectively, greater than the threshold for species demarcation, confirming that they represent the same species. Relatively low dDDH values (less than the 79-80 % threshold for subspecies demarcation) between the type strains of and indicated that can be considered as a subspecies of . On the basis of the results presented here, (i) and should not be transferred to ; (ii) we propose Biavati 1992 as a later heterotypic synonym of Watabe 1983 and as a new subspecies of , for which the name subsp. subsp. nov. is proposed.
2018 年，Nouioui 根据数字 DNA-DNA 杂交（dDDH）值将 和 转移到 subsp. 和 subsp.。这两个新亚种同年得到了验证。然而，我们发现 Nouioui 在 dDDH 分析中使用的 基因组（GenBank/ENA/DDBJ 登录号 JGZJ01000000）不能代表 。因此， 和 之间的分类关系需要重新审查。DSM 20433 与 和 的模式株的平均核苷酸同一性（ANI）值分别为 88.7-89.0%和 37.5-38.0%，低于种间区分的阈值，证实 代表与 和 不同的种。 和 模式株之间的 ANI 值和 dDDH 值分别为 96.7-96.9%和 73.0-73.3%，大于种间区分的阈值，证实它们代表相同的种。 和 模式株之间相对较低的 dDDH 值（低于亚种区分的 79-80%阈值）表明 可以被视为 的一个亚种。基于这里提出的结果，（i） 和 不应转移到 ；（ii）我们提议将 1992 年的 Biavati 作为 1983 年 Watabe 的异名，并作为 的一个新亚种，提议使用 subsp. subsp. nov. 的名称。
