Department of Microbiology, Nutrition and Dietetics, Czech University of Life Sciences Prague, Kamýcká 129, Prague 6 - Suchdol, 165 00, Czechia.
Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability, University of Parma, Parma, Italy.
Int J Syst Evol Microbiol. 2021 Jan;71(1). doi: 10.1099/ijsem.0.004573. Epub 2020 Nov 23.
Five strains, VB23, VB24, VB25, VB26 and VB31, were isolated from chimpanzee (), cotton-top tamarin (), Goeldi's marmoset (), moustached tamarin () and patas monkey (), respectively, which were kept in two Czech zoos. These strains were isolated from faecal samples and were Gram-positive, non-motile, non-sporulating, anaerobic and fructose-6-phosphate phosphoketolase-positive. Phylogenetic analyses based on 16S rRNA revealed close relatedness between VB23 and LMG 11039 (96.0 %), VB24 and subsp. DSM 20433 (96.1 %), VB25 and LMG 30939 (96.5 %), VB26 and LMG 30297 (98.1 %), and VB31 and . LMG 11039 (99.40 %). Internal transcribed spacer profiling revealed that VB23, VB24, VB25, VB26 and VB31 had highest similarity to LMG 13208 (77.2 %), subsp. ATCC 15697 (85.8 %), DSM 23969 (76.9 %), LMG 13208 (81.2 %) and LMG 11039 (88.2 %), respectively. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with their closest neighbours supported the independent phylogenetic positions of the strains with values between 86.3 and 94.3 % for ANI and 25.8 and 54.9 % for dDDH. These genomic and phylogenetic analyses suggested that the evaluated strains were novel species named sp. nov. (VB31=DSM 109960=CCUG 73843), sp. nov. (VB25=DSM 109958=CCUG 73842), sp. nov. (VB24=DSM 109957=CCUG 73932), sp. nov. (VB26=DSM 109959=CCUG 73845) and sp. nov. (VB23=DSM 109963=CCUG 73840).
从分别饲养在捷克两个动物园的黑猩猩（）、棉顶狨猴（）、金狮面狨（）、毛丝鼠（）和黑脸长尾猴（）的粪便样本中分离到 5 株菌，VB23、VB24、VB25、VB26 和 VB31，它们分别被命名为 VB23、VB24、VB25、VB26 和 VB31。这些菌株均为革兰氏阳性、非运动性、非孢子形成、厌氧和果糖-6-磷酸磷酸酮醇酶阳性。基于 16S rRNA 的系统发育分析表明 VB23 与 LMG 11039（96.0%）、VB24 与 subsp. DSM 20433（96.1%）、VB25 与 LMG 30939（96.5%）、VB26 与 LMG 30297（98.1%）和 VB31 与. LMG 11039（99.40%）密切相关。内部转录间隔区分析显示 VB23、VB24、VB25、VB26 和 VB31 与 LMG 13208（77.2%）、 subsp. ATCC 15697（85.8%）、 DSM 23969（76.9%）、 LMG 13208（81.2%）和 LMG 11039（88.2%）具有最高的相似性。与最接近的近缘种的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）分析支持这些菌株的独立进化地位，ANI 值在 86.3%到 94.3%之间，dDDH 值在 25.8%到 54.9%之间。这些基因组和系统发育分析表明，评估的菌株是新的 种，命名为 sp. nov.（VB31=DSM 109960=CCUG 73843）、 sp. nov.（VB25=DSM 109958=CCUG 73842）、 sp. nov.（VB24=DSM 109957=CCUG 73932）、 sp. nov.（VB26=DSM 109959=CCUG 73845）和 sp. nov.（VB23=DSM 109963=CCUG 73840）。
