Chien Ju-Huei, Lee Shan-Chih, Chang Kai-Fu, Huang Xiao-Fan, Chen Yi-Ting, Tsai Nu-Man
Department of Laboratory Medicine, Taichung Tzu-Chi Hospital, Buddhist Tzu-Chi Medical Foundation, Taichung 42743, Taiwan.
Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan.
Evid Based Complement Alternat Med. 2020 Sep 9;2020:9758156. doi: 10.1155/2020/9758156. eCollection 2020.
(PCa), an herb used in traditional Chinese medicine, is routinely used in the amelioration of different types of gastrointestinal discomfort. However, the mechanisms underlying the cancer suppression activity of PCa in colorectal cancer (CRC) cells have yet to be clarified. The aim of this study was to investigate the anticancer effects of PCa, specifically the induction of apoptosis in CRC cells. The growth inhibition curve of CRC cells following exposure to PCa was detected by an MTT assay. Moreover, PCa combined with 5-FU revealed a synergic effect of decreased cell viability. PCa inhibited cell proliferation and induced cell cycle arrest at the G/G phase and cell apoptosis through regulation of associated protein expression. An in vivo study showed that PCa suppressed the growth of CRC via induction of cell apoptosis with no significant change in body weight or organ histology. Our results demonstrated that PCa inhibits the growth of CRC cells and induces apoptosis in vitro and in vivo, which suggests the potential applicability of PCa as an anticancer agent.
马齿苋（PCa）是一种用于传统中药的草药，常用于缓解不同类型的胃肠道不适。然而，马齿苋在结直肠癌（CRC）细胞中抑制癌症活性的潜在机制尚未阐明。本研究的目的是研究马齿苋的抗癌作用，特别是其诱导CRC细胞凋亡的作用。通过MTT法检测CRC细胞暴露于马齿苋后的生长抑制曲线。此外，马齿苋与5-氟尿嘧啶联合使用显示出降低细胞活力的协同作用。马齿苋通过调节相关蛋白表达抑制细胞增殖并诱导细胞周期停滞在G/G期以及细胞凋亡。一项体内研究表明，马齿苋通过诱导细胞凋亡抑制CRC的生长，而体重或器官组织学无显著变化。我们的结果表明，马齿苋在体外和体内均能抑制CRC细胞的生长并诱导凋亡，这表明马齿苋作为抗癌剂具有潜在的适用性。
