Chien Ju-Huei, Huang Xiao-Fan, Lai Wen-Lin, Chang Kai-Fu, Li Chia-Yu, Chen Szu-Yin, Wu Chun-Yu, Li Kuan-Ying, Tsai Nu-Man
Department of Laboratory Medicine Taichung Tzu-Chi Hospital Buddhist Tzu-Chi Medical Foundation Taichung Taiwan, ROC.
Department of Medical Laboratory Science and Biotechnology Central Taiwan University of Science and Technology Taichung Taiwan, ROC.
Food Sci Nutr. 2021 May 2;9(6):3209-3218. doi: 10.1002/fsn3.2282. eCollection 2021 Jun.
has been indicated to treat many kinds of diseases and the progression of cancers, such as colorectal cancer. However, the effects of extract (PPa extract) against acute myeloid leukemia have not been investigated. Thus, this study explored the anticancer potential of PPa extract and its mechanism in HL-60 cells. The MTT assay results showed that PPa extract significantly inhibited the proliferation of HL-60 cells in a dose-dependent manner and affected cell morphology, causing cell shrinkage and the formation of debris. PPa extract blocked cell cycle progression at the G/G phase in a dose- and time-dependent manner and induced cell apoptosis, as shown by the observation of DNA fragments and apoptotic bodies. Furthermore, PPa extract caused the accumulation of a population of cells at G/G phase via a reduction in p-Rb, increasing p21 expression, and downregulating cell cycle regulator protein expression. Then, PPa extract was found to activate the extrinsic and intrinsic apoptosis pathways, leading to cell death. These data demonstrated that PPa extract exerted inhibitory activity and triggered cell apoptosis in HL-60 cells and that PPa extract might be a chemopreventive agent for cancer therapy.
已被证明可治疗多种疾病及癌症进展，如结直肠癌。然而，[提取物名称]提取物（PPa提取物）对急性髓系白血病的作用尚未得到研究。因此，本研究探讨了PPa提取物在HL - 60细胞中的抗癌潜力及其机制。MTT试验结果表明，PPa提取物以剂量依赖性方式显著抑制HL - 60细胞的增殖，并影响细胞形态，导致细胞收缩和碎片形成。如通过观察DNA片段和凋亡小体所示，PPa提取物以剂量和时间依赖性方式阻断细胞周期在G/G期的进展并诱导细胞凋亡。此外，PPa提取物通过降低p - Rb、增加p21表达和下调细胞周期调节蛋白表达，导致一群细胞在G/G期积累。然后，发现PPa提取物激活外源性和内源性凋亡途径，导致细胞死亡。这些数据表明，PPa提取物在HL - 60细胞中发挥抑制活性并触发细胞凋亡，并且PPa提取物可能是一种用于癌症治疗的化学预防剂。
