Banikamali Mania, Soltanloo Hassan, Ramezanpour S Sanaz, Yamchi Ahad, Sorahinobar Mona
Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Golestan, Iran.
Department of Plant Biology, Faculty of Biological Science, Alzahra University, Tehran, Iran.
Biotechnol Rep (Amst). 2020 Aug 11;28:e00520. doi: 10.1016/j.btre.2020.e00520. eCollection 2020 Dec.
Currently, a global demand exists forlavender as a significant medicinal plant and source of essential oils. Freshwater and arable lands are two major factors that inhibit extensive farming of medicinal plants in Iran. Saline water from seas and salty soil may be new resources for agricultural use, especially for medicinal plants. We sought to extend our knowledge of the genome and molecular basis of its salinity tolerance by using cDNA amplified fragment length polymorphism (cDNA-AFLP) to investigate the changes in plant transcriptomes in response to NaCl. All identified transcript derived fragments (TDF) were assigned as novel genes related to signal transduction, regulation of gene expression, alternative splicing, autophagy, and secondary metabolite biosynthesis. qRT-PCR analysis of the TDFs in response to different concentrations of NaCl revealed various levels of mRNA of the identified genes in this plant. Our findings provided primary insights into the molecular response of to salinity.
目前，全球对薰衣草作为一种重要的药用植物和精油来源存在需求。淡水和耕地是阻碍伊朗药用植物广泛种植的两个主要因素。海水和盐碱土中的咸水可能是农业利用的新资源，特别是对于药用植物。我们试图通过使用cDNA扩增片段长度多态性（cDNA-AFLP）来研究植物转录组对NaCl的响应变化，从而扩展我们对其耐盐性的基因组和分子基础的认识。所有鉴定出的转录衍生片段（TDF）都被归类为与信号转导、基因表达调控、可变剪接、自噬和次生代谢物生物合成相关的新基因。对不同浓度NaCl响应的TDF进行qRT-PCR分析，揭示了该植物中鉴定出的基因的不同水平的mRNA。我们的研究结果为其对盐度的分子响应提供了初步见解。
