Fatemi Farzaneh, Hashemi-Petroudi Seyyed Hamidreza, Nematzadeh Ghorbanali, Askari Hossein, Abdollahi Mohammad Reza
1Department of Genetic Engineering and Molecular Biology, Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), P.O. Box 578, Sari, Iran.
3Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
Biol Proced Online. 2019 Jul 15;21:14. doi: 10.1186/s12575-019-0103-3. eCollection 2019.
Salinity as a most significant environmental challenges affects the growth and productivity of plants worldwide. In this study, the ionic and iso-osmotic effects of salt stress were investigated in L., a halophyte grass species from Poaceae family, by cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique. To dissect the two different effects (ionic and osmotic) exerted by salt stress, various ionic agents including 200 and 400 mM sodium chloride (NaCl), 200 and 400 mM potassium chloride (KCl) as well as 280 and 406 gl (- 0.9 and - 1.4 MPa) polyethylene glycol 6000 (PEG) as their iso-osmotic concentrations were applied.
Application of KCl and PEG significantly reduced the fresh weight (FW) of seedlings compared to control while NaCl treatment markedly enhanced the FW. At the transcriptome level, different observations of changes in gene expression have been made in response of to ionic and osmotic stresses. Out of 69 transcript derived fragments (TDFs), 42 TDFs belong to 9 different groups of genes involved in metabolism (11.6%), transcription (10.2%), ribosomal protein (8.7%), protein binding (8.7%) transporter (5.8%), translation (5.8%), signal transduction (4.3%), nucleosome assembly protein (2.9%) and catabolism (2.9%). The 44 and 28 percent of transcripts were expressed under ionic stress (NaCl-specific and KCl-specific) and osmotic stress (common with NaCl, KCl and PEG), respectively which indicating a greater response of plants to ionic stress than osmotic stress. Expression pattern of eight candidate TDFs including; , , , , , , and was evaluated by RT-qPCR at high salinity levels and recovery condition.
Differential regulation of these TDFs was observed in root and shoot which confirm their role in salt stress tolerance and provide initial insights into the transcriptome of . Expression pattern of ionic and osmotic-related TDFs at can be taken as an indication of their functional relevance at different salt and drought stresses.
盐度作为一个最显著的环境挑战，影响着全球植物的生长和生产力。在本研究中，通过cDNA扩增片段长度多态性（cDNA-AFLP）技术，对禾本科盐生草种盐地碱蓬中盐胁迫的离子效应和等渗效应进行了研究。为了剖析盐胁迫施加的两种不同效应（离子效应和渗透效应），应用了各种离子剂，包括200和400 mM氯化钠（NaCl）、200和400 mM氯化钾（KCl）以及280和406 g l（-0.9和-1.4 MPa）聚乙二醇6000（PEG）作为它们的等渗浓度。
与对照相比，KCl和PEG的应用显著降低了盐地碱蓬幼苗的鲜重（FW），而NaCl处理显著提高了FW。在转录组水平上，针对盐地碱蓬对离子胁迫和渗透胁迫的响应，对基因表达变化进行了不同的观察。在69个转录衍生片段（TDF）中，42个TDF属于9个不同的基因组，涉及代谢（11.6%）、转录（10.2%）、核糖体蛋白（8.7%）、蛋白结合（8.7%）、转运蛋白（5.8%）、翻译（5.8%）、信号转导（4.3%）、核小体组装蛋白（2.9%）和分解代谢（2.9%）。分别有44%和28%的转录本在离子胁迫（NaCl特异性和KCl特异性）和渗透胁迫（与NaCl、KCl和PEG共同）下表达，这表明植物对离子胁迫的响应大于对渗透胁迫的响应。在高盐度水平和恢复条件下，通过RT-qPCR对包括……在内的8个候选TDF的表达模式进行了评估。
在根和茎中观察到这些TDF的差异调节，这证实了它们在耐盐胁迫中的作用，并为盐地碱蓬的转录组提供了初步见解。盐地碱蓬中离子和渗透相关TDF的表达模式可作为它们在不同盐胁迫和干旱胁迫下功能相关性的一个指标。
