基于海藻酸钠保护的银纳米粒子的表面增强拉曼散射快速测定丙硫氧嘧啶和甲巯咪唑。

Rapid determination of propylthiouracil and methimazole by surface-enhanced Raman scattering based on sodium alginate-protected silver nanoparticles.

机构信息

College of Chemistry, Jilin University, Changchun, 130012, Jilin, China.

State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, 130012, Jilin, China.

出版信息

Anal Bioanal Chem. 2020 Nov;412(28):7827-7836. doi: 10.1007/s00216-020-02912-1. Epub 2020 Sep 22.

DOI:10.1007/s00216-020-02912-1

PMID:32964253

Abstract

A rapid, ultrasensitive and selective method has been established to determine antithyroid drugs [propylthiouracil (PTU) and methimazole (MTZ)] by surface-enhanced Raman scattering (SERS). Silver nanoparticles (AgNPs) were synthesized by reduction of silver nitrate (AgNO) with sodium alginate (SA) and were used as SERS substrates. Through optimization of the SERS conditions, including the volume of SA-protected AgNPs solution, pH of Britton-Robinson (BR) buffer solution and concentration of NaCl solution, linear responses were obtained for PTU and MTZ in the concentration ranges of 3.02 × 10-1.06 × 10 mol L and 1.21 × 10-1.21 × 10 mol L, respectively. By the present method, the limits of detection (LODs) for the determination of PTU and MTZ were as low as 1.58 × 10 mol L and 2.97 × 10 mol L. The method was successfully applied for the determination of PTU and MTZ in real samples. The recovery of PTU and MTZ from actual samples ranged between 97.20 and 104.80%, with relative standard deviations (RSDs) less than 3.63%.

摘要

建立了一种快速、灵敏和选择性的方法，通过表面增强拉曼散射（SERS）来测定抗甲状腺药物[丙基硫氧嘧啶（PTU）和甲巯咪唑（MTZ）]。通过用海藻酸钠（SA）还原硝酸银（AgNO）合成了银纳米粒子（AgNPs），并将其用作 SERS 基底。通过优化 SERS 条件，包括 SA 保护的 AgNPs 溶液的体积、Britton-Robinson（BR）缓冲溶液的 pH 和 NaCl 溶液的浓度，分别获得了 PTU 和 MTZ 在 3.02×10-1.06×10 mol L 和 1.21×10-1.21×10 mol L 的浓度范围内的线性响应。通过本方法，PTU 和 MTZ 的检测限（LOD）分别低至 1.58×10 mol L 和 2.97×10 mol L。该方法成功应用于实际样品中 PTU 和 MTZ 的测定。PTU 和 MTZ 从实际样品中的回收率在 97.20%至 104.80%之间，相对标准偏差（RSD）小于 3.63%。