药物、动物饲料和动物肝脏中卡比马唑、甲巯咪唑和丙硫氧嘧啶的动力学测定

Kinetic determination of carbimazole, methimazole and propylthiouracil in pharmaceuticals, animal feed and animal livers.

作者信息

Soledad García M, Albero M I, Sánchez-Pedreño C, Tobal L

机构信息

Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.

出版信息

Analyst. 1995 Jan;120(1):129-33. doi: 10.1039/an9952000129.

DOI:10.1039/an9952000129

PMID:7710122

Abstract

Kinetic methods are proposed for the determination of carbimazole(CBZ), methimazole(MMI) and propylthiouracil(PTU) based on their inhibitory effects on the palladium(II)-catalysed reaction between the pyronine G and hypophosphite ions. The reactions are monitored spectrophotometrically at 548 nm. The influence of reaction variables and the interfering effect of other substances were studied. Under the selected experimental conditions (CBZ: 3.6 x 10(-5) mol l-1 pyronine G; pH 3.0; 0.7 microgram ml-1 PdII and 0.2 mol l-1 H2PO2-; MMI: 6 x 10(-5) mol l-1 pyronine G, pH 3.0; 0.7 microgram ml-1 PdII, 0.4 mol l-1 H2PO2-; PTU: 3.6 x 10(-5) mol l-1 pyronine G; pH 3.0; 0.7 microgram ml-1 PdII and 0.3 mol l-1 H2PO2-), these three antithyroids were determined in the following ranges: CBZ, 0.04-0.70 microgram ml-1; MMI, 0.04-0.50 microgram ml-1; PTU 0.08-1.00 microgram ml-1. The methods were applied to the determination of CBZ, MMI and PTU, in pharmaceuticals, animal feed and animal livers.

摘要

基于卡比马唑（CBZ）、甲巯咪唑（MMI）和丙硫氧嘧啶（PTU）对钯（II）催化的焦宁G与次磷酸根离子之间反应的抑制作用，提出了动力学方法用于测定这三种物质。反应在548nm处用分光光度法进行监测。研究了反应变量的影响以及其他物质的干扰作用。在选定的实验条件下（CBZ：3.6×10⁻⁵mol·l⁻¹焦宁G；pH 3.0；0.7μg·ml⁻¹ PdII和0.2mol·l⁻¹ H₂PO₂⁻；MMI：6×10⁻⁵mol·l⁻¹焦宁G，pH 3.0；0.7μg·ml⁻¹ PdII，0.4mol·l⁻¹ H₂PO₂⁻；PTU：3.6×10⁻⁵mol·l⁻¹焦宁G；pH 3.0；0.7μg·ml⁻¹ PdII和0.3mol·l⁻¹ H₂PO₂⁻），这三种抗甲状腺药物的测定范围如下：CBZ为0.04 - 0.70μg·ml⁻¹；MMI为0.04 - 0.50μg·ml⁻¹；PTU为0.08 - 1.00μg·ml⁻¹。这些方法已应用于药物、动物饲料和动物肝脏中CBZ、MMI和PTU的测定。