Department of Chemical Sciences, University of Naples Federico II Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126, Napoli, Italy.
Present address: Department of Organic Chemistry, Stockholm University, Svante Arrhenius väg 16C, 106 91, Stockholm, Sweden.
Chembiochem. 2021 Jan 5;22(1):147-150. doi: 10.1002/cbic.202000597. Epub 2020 Oct 29.
Acetobacter pasteurianus, a member of the Alphaproteobacteria, is an acetic acid-producing bacterium present on sugar-rich substrates such as such as fruits, flowers and vegetables and traditionally used in the production of fermented food. The preferred living habitat associated with acid conditions makes the structure of the bacterial cell wall interesting to study, due to expected uncommon features. We have used a combination of chemical, analytical and NMR spectroscopy approaches to define the complete structure of the core oligosaccharide from A. pasteurianus CIP103108 LPS. Interestingly, the core oligosaccharide displays a high concentration of negatively charged groups, structural features that might contribute to reinforcing the bacterial membrane.
醋杆菌，α变形菌的一个成员，是一种产生乙酸的细菌，存在于富含糖分的基质上，如水果、花卉和蔬菜，并被传统上用于发酵食品的生产。与酸性条件相关的首选生活环境使得研究细菌细胞壁的结构变得有趣，因为预计会有不常见的特征。我们使用化学、分析和 NMR 光谱学方法的组合来定义 A. pasteurianus CIP103108 LPS 的核心寡糖的完整结构。有趣的是，核心寡糖显示出高浓度的带负电荷的基团，这些结构特征可能有助于增强细菌膜。
