使用共聚焦显微镜和相差显微镜对巨型单层囊泡进行波动光谱分析。
Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy.
作者信息
Faizi Hammad A, Reeves Cody J, Georgiev Vasil N, Vlahovska Petia M, Dimova Rumiana
机构信息
Department of Mechanical Engineering, McCormick School of Engineering and Applied Science, Northwestern University, Evanston, Illinois 60208, USA.
Department of Engineering Sciences and Applied Mathematics, McCormick School of Engineering and Applied Science, Northwestern University, Evanston, Illinois 60208, USA.
出版信息
Soft Matter. 2020 Sep 23. doi: 10.1039/d0sm00943a.
A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.
一种广泛用于测量双层膜弯曲刚度的方法是波动光谱法，该方法分析通过相差显微镜或共聚焦显微镜记录的巨型单层囊泡的热驱动膜波动。在这里，我们使用这两种技术分析同一个囊泡的波动，并获得了弯曲模量的一致值。我们讨论了可能导致差异的因素。
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