Li Liyang, Zhang Jiaming, Li Zhuozhe, Zhang Cuiping, Bi Jing, Zhou Jian, Song Yuanlin, Shao Changzhou
Shanghai Respiratory Research Institute, Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
College of Life Science, Shandong Normal University, Jinan, China.
Clin Respir J. 2021 Feb;15(2):154-162. doi: 10.1111/crj.13279. Epub 2020 Oct 14.
Airway microbiota are associated with several chronic respiratory diseases. However, limited studies examined microbiota in non-cystic fibrosis (non-CF) bronchiectasis, especially its relationship with severity and immunology.
We characterized the microbiota of patients with different severities of bronchiectasis and examined the correlation between microbiota and the immunological indices.
The microbiota of 63 sputum samples from 40 patients with bronchiectasis were analyzed by 16S rRNA gene sequencing. Blood tests and related immunological indices were detected.
According to the baseline data of patients with bronchiectasis, we found that more severe bronchiectasis was accompanied by lower prealbumin levels. The 16S rRNA sequencing analyses demonstrated that Pseudomonas-dominated microbiota produced lower levels of interleukin-4 (IL-4) and transforming growth factor-β (TGF-β) compared to other-dominated microbiota. The airway microbiota of patients with mild bronchiectasis clustered apart from those of patients with severe bronchiectasis, which correlated with IL-4 and other clinical indices.
There are differences in the airway microbiota between patients with mild and severe bronchiectasis. The airway microbiota was related to some clinical indices that represent effects on the immune system.
气道微生物群与多种慢性呼吸道疾病相关。然而，针对非囊性纤维化（非CF）支气管扩张症中微生物群的研究有限，尤其是其与疾病严重程度和免疫学的关系。
我们对不同严重程度支气管扩张症患者的微生物群进行了特征分析，并检测了微生物群与免疫指标之间的相关性。
采用16S rRNA基因测序分析40例支气管扩张症患者63份痰液样本中的微生物群。进行血液检测并检测相关免疫指标。
根据支气管扩张症患者的基线数据，我们发现支气管扩张症越严重，前白蛋白水平越低。16S rRNA测序分析表明，与其他微生物群主导的情况相比，以铜绿假单胞菌为主导的微生物群产生的白细胞介素-4（IL-4）和转化生长因子-β（TGF-β）水平较低。轻度支气管扩张症患者的气道微生物群与重度支气管扩张症患者的气道微生物群聚类不同，这与IL-4和其他临床指标相关。
轻度和重度支气管扩张症患者的气道微生物群存在差异。气道微生物群与一些代表对免疫系统影响的临床指标有关。
