College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
J Pharm Biomed Anal. 2020 Nov 30;191:113609. doi: 10.1016/j.jpba.2020.113609. Epub 2020 Sep 13.
Different therapeutically active ingredients, from plants, animals, and mineral sources, are prescribed as traditional Chinese medicines (TCM). TCMs, from animal sources, are rich in proteins and peptides. Different advanced proteomics technologies, such as two-dimensional gel electrophoresis (2-DE), multi-dimensional liquid chromatography (MDLC), matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS), and isobaric tags for relative and absolute quantitation (iTRAQ), have been applied to analyze TCMs, from animal sources. This paper reviews the common proteomic techniques for analyzing animal - derived TCMs. Various scientific studies have reported the application of proteomics for locating drug targets, identifying active components, and elucidating the mechanisms of action of animal - derived TCMs. However, these researches are still at the preliminary stage. This review has also discussed the existing challenges and future directions in this field of research.
不同的治疗活性成分，来自植物、动物和矿物来源，被规定为中药（TCM）。来自动物来源的 TCM 富含蛋白质和肽。不同的先进蛋白质组学技术，如二维凝胶电泳（2-DE）、多维液相色谱（MDLC）、基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）和相对和绝对定量的等压标签（iTRAQ），已被应用于分析来自动物来源的 TCM。本文综述了分析动物源性 TCM 的常用蛋白质组学技术。各种科学研究报告了蛋白质组学在定位药物靶点、鉴定活性成分和阐明动物源性 TCM 作用机制方面的应用。然而，这些研究仍处于初步阶段。本文还讨论了该领域研究中存在的挑战和未来方向。
