Runel Gaël, Cario Muriel, Lopez-Ramirez Noémie, Malbouyres Marilyne, Ruggiero Florence, Bernard Laure, Puisieux Alain, Caramel Julie, Chlasta Julien, Masse Ingrid
Centre de Recherche en Cancérologie de Lyon, CNRS UMR5286, Inserm U1052, Université Lyon 1, Université de Lyon, Lyon, France.
BioMeca, Lyon, France.
Exp Dermatol. 2020 Dec;29(12):1233-1237. doi: 10.1111/exd.14195. Epub 2020 Oct 12.
Human skin is particularly vulnerable to age-related deterioration and undergoes profound structural and functional changes, reflected in the external skin appearance. Skin ageing is characterized by features such as wrinkling or loss of elasticity. Even if research advances have been done concerning the molecular mechanisms that underlie these changes, very few studies have been conducted concerning the structure stiffness of the skin organ as a whole. In this study, we showed, thanks to human skin reconstructs and the Japanese Medaka fish model, that biomechanics is a new biomarker of skin ageing. We revealed that global stiffness measurement by Atomic Force Microscopy, since modulated through ageing in these models, can be a new biomarker of skin ageing, and reflects the profound reorganization of the dermis extracellular matrix, as shown by Transmission Electron Microscopy. Moreover, our data unveiled that the Japanese Medaka fish could represent a highly relevant integrated model to study skin ageing in vivo.
人类皮肤特别容易受到与年龄相关的退化影响，并经历深刻的结构和功能变化，这在皮肤外观上有所体现。皮肤老化的特征包括皱纹或弹性丧失等。尽管在这些变化背后的分子机制方面已有研究进展，但关于整个皮肤器官的结构硬度的研究却很少。在本研究中，借助人类皮肤重建模型和日本青鳉鱼模型，我们表明生物力学是皮肤老化的一种新生物标志物。我们发现，通过原子力显微镜进行的整体硬度测量，由于在这些模型中会随年龄变化而受到调节，可成为皮肤老化的一种新生物标志物，并反映真皮细胞外基质的深刻重组，这在透射电子显微镜下得到了证实。此外，我们的数据表明，日本青鳉鱼可能是研究体内皮肤老化的一个高度相关的综合模型。
