百里醌对叔丁基对苯二酚诱导的人脐静脉内皮细胞毒性的保护作用。
The protective effect of thymoquinone on -butylhydroquinone induced cytotoxicity in human umbilical vein endothelial cells.
作者信息
Karimi Zahra, Ghaffari Maryam, Ezzati Nazhad Dolatabadi Jafar, Dehghan Parvin
机构信息
Immunology Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
Department of Food Science and Technology , Nutrition Research Center , Faculty of Nutrition and Food Sciences , Tabriz University of Medical Sciences , Tabriz , Iran . Email:
出版信息
Toxicol Res (Camb). 2019 Nov 29;8(6):1050-1056. doi: 10.1039/c9tx00235a. eCollection 2019 Nov 1.
2--Butyl-4-hydroquinone (TBHQ) is used for inhibition of oxidative rancidity in the food industry. However, this antioxidant can stimulate cytotoxicity in human umbilical vein endothelial cells (HUVECs). Thus, potential protective effects of thymoquinone (TQ) against TBHQ-induced cytotoxicity were investigated. Cytotoxicity was evaluated MTT, flow cytometry, DAPI staining and DNA fragmentation methods. The obtained results revealed that treatment of HUVECs with TQ enhanced the cell viability rate and it had potential to reduce the cytotoxicity effect of TBHQ in cells. Also, in a combined regime of TQ and TBHQ, apoptosis was reduced compared to the cells treated with TBHQ ( < 0.05). Similarly, TQ had a protective effect on DNA and chromatin fragmentation of the cells treated with TBHQ. Finally, it can be concluded that TQ could be used as a protective agent against cytotoxicity induced by TBHQ in HUVECs.
2-叔丁基对苯二酚（TBHQ）在食品工业中用于抑制氧化酸败。然而，这种抗氧化剂会刺激人脐静脉内皮细胞（HUVECs）产生细胞毒性。因此，研究了百里醌（TQ）对TBHQ诱导的细胞毒性的潜在保护作用。采用MTT、流式细胞术、DAPI染色和DNA片段化方法评估细胞毒性。所得结果表明，用TQ处理HUVECs可提高细胞活力率，并有可能降低TBHQ对细胞的细胞毒性作用。此外，在TQ和TBHQ的联合处理组中，与用TBHQ处理的细胞相比，细胞凋亡减少（P<0.05）。同样，TQ对用TBHQ处理的细胞的DNA和染色质片段化具有保护作用。最后，可以得出结论，TQ可作为一种保护剂，防止TBHQ在HUVECs中诱导的细胞毒性。
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