对从七个受感染叶片样本中获得的逆转录聚合酶链反应（RT-PCR）产物进行的序列分析表明，印度水仙（Narcissus tazetta L.）中存在三种马铃薯Y病毒属病毒。
Sequence analyses of RT-PCR products obtained from seven infected leaf samples revealed existence of three potyvirus species in Indian narcissus ( L.).
作者信息
Raj Rashmi, Kaur Charanjeet, Srivastava Ashish, Kumar Susheel, Raj S K
机构信息
Plant Virus Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001 India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, UP 201002 India.
出版信息
3 Biotech. 2020 Oct;10(10):428. doi: 10.1007/s13205-020-02418-8. Epub 2020 Sep 11.
Abstract
Potyvirus species associated with yellow leaf stripe disease of Indian narcissus ( L.) var. Paperwhite has been studied by sequence analyses of ~ 1.5 kb genomic fragments obtained from seven RT-PCR amplifications of infected samples. Sequence analysis revealed the occurrence of three potyvirus species: A (CEVA: KF430815, KF430816, KM066973, KM066974); (NYSV: KM066972, JQ686724) and (NDV: MK572806). The existence of three potyvirus species: CEVA, NYSV and NDV are being reported in Indian narcissus.
摘要
通过对感染样本进行7次逆转录聚合酶链反应（RT-PCR）扩增得到的约1.5 kb基因组片段进行序列分析，对与印度水仙（L.）品种白花水仙黄叶条纹病相关的马铃薯Y病毒属病毒种类进行了研究。序列分析揭示了三种马铃薯Y病毒属病毒种类的存在：A（CEVA：KF430815、KF430816、KM066973、KM066974）；（NYSV：KM066972、JQ686724）和（NDV：MK572806）。印度水仙中存在三种马铃薯Y病毒属病毒：CEVA、NYSV和NDV，这一情况在此予以报道。
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