紫草毛状根培养体系的建立及其紫草素的生产。
Establishment of the hairy root culture of L. and its shikonin production.
作者信息
Fu Jiang-Yan, Zhao Hua, Bao Jia-Xin, Wen Zhong-Ling, Fang Rong-Jun, Fazal Aliya, Yang Min-Kai, Liu Bao, Yin Tong-Ming, Pang Yan-Jun, Lu Gui-Hua, Qi Jin-Liang, Yang Yong-Hua
机构信息
Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023 China.
Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, 130024 China.
出版信息
3 Biotech. 2020 Oct;10(10):429. doi: 10.1007/s13205-020-02419-7. Epub 2020 Sep 11.
L. (Boraginaceae) is an invasive species in Australia and contains medicinal shikonins in its roots. In this study, the hairy root lines of were established using strain ATCC15834 and confirmed by the amplification of the gene. Results showed significant difference in shikonin production between the hairy root lines in the /B5 and M9 media. The biomass of the lines in the /B5 medium was fivefold of that in the M9 medium. However, the components of detected shikonins were similar in these two liquid media. By contrast, different accumulation profiles appeared in the hairy root lines. HPLC analysis revealed the presence of nine possible related compounds, including shikonins, and acetylshikonin was the most abundant shikonin derivative. The content of acetylshikonin in the /B5 medium (36.25 mg/L on average) was twofold of that in the M9 medium. Our results showed that the hairy root cultures of can be used in enhancing the production of potential pharmaceutical compounds, such as acetylshikonin.
L.（紫草科）是澳大利亚的一种入侵物种，其根部含有药用紫草素。在本研究中，使用发根农杆菌ATCC15834建立了L.的毛状根系，并通过扩增rolC基因进行了确认。结果表明，在/B5和M9培养基中，毛状根系之间紫草素的产量存在显著差异。/B5培养基中毛状根系的生物量是M9培养基中的五倍。然而，在这两种液体培养基中检测到的紫草素成分相似。相比之下，毛状根系中出现了不同的积累模式。高效液相色谱分析显示存在九种可能的相关化合物，包括紫草素，乙酰紫草素是最丰富的紫草素衍生物。/B5培养基中乙酰紫草素的含量（平均36.25mg/L）是M9培养基中的两倍。我们的结果表明，L.的毛状根培养可用于提高潜在药物化合物如乙酰紫草素的产量。
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