Department of Pediatric Surgery, Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266000, China.
Department of Pediatric Surgery, Yijishan Hospital of Wannan Medical College, Wannan Medical College, Wuhu 246400, China.
Fetal Pediatr Pathol. 2022 Jun;41(3):371-380. doi: 10.1080/15513815.2020.1822965. Epub 2020 Sep 24.
We investigated three-dimensional (3 D) reconstruction for the assessment of the tumor margin microstructure of hepatoblastoma (HB). Eleven surgical resections of childhood hepatoblastomas obtained between September 2018 and December 2019 were formalin-fixed, paraffin-embedded, serially sectioned at 4 μm, stained with hematoxylin and eosin (every 19th and 20th section stained with alpha-fetoprotein and glypican 3), and the digital images of all sections were acquired at 100× followed by image registration using the B-spline based method with modified residual complexity. Reconstruction was performed using 3 D Slicer software. The reconstructed orthogonal 3 D images clearly presented the internal microstructure of the tumor margin. The rendered 3 D image could be rotated at any angle. Microstructure 3 D reconstruction is feasible for observing the pathological structure of the HB tumor margin.
我们研究了三维（3D）重建，以评估肝母细胞瘤（HB）的肿瘤边缘微观结构。 2018 年 9 月至 2019 年 12 月期间，我们对 11 例儿童肝母细胞瘤的手术切除标本进行了福尔马林固定、石蜡包埋、4μm 连续切片、苏木精和伊红（每 19 个和 20 个切片用甲胎蛋白和糖蛋白 3 染色）染色，并在 100×下获取所有切片的数字图像，然后使用基于 B 样条的方法和修改后的残差复杂度进行图像配准。使用 3D Slicer 软件进行重建。重建的正交 3D 图像清晰地呈现了肿瘤边缘的内部微观结构。渲染的 3D 图像可以任意角度旋转。3D 微观结构重建可用于观察 HB 肿瘤边缘的病理结构。
