Federal University of Grande Dourados (UFGD), Dourados79825-070, Brazil.
Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP13630-970, Brazil.
Zygote. 2021 Feb;29(1):82-86. doi: 10.1017/S096719942000043X. Epub 2020 Sep 24.
The aim of this study was to evaluate different post-shock temperatures for tetraploid induction in the yellowtail tetra Astyanax altiparanae. Newly fertilized eggs were divided into four groups, three were submitted to heat shock (40°C for 2 min) at 24 min post-fertilization (mpf) and another group remained without shock (control). Groups submitted to temperature shock were further separated at the following temperatures: 22°C, 26°C and 28°C. Survival among embryonic development was counted and at hatching the ploidy was analyzed by flow cytometry. The results showed that the post-shock temperature affects the parameters analyzed and, therefore, must be considered for optimization of the production of tetraploid in A. altiparanae. Those data are innovative and could be used in future studies of basic biology in this species.
本研究旨在评估黄尾脂鲤（Astyanax altiparanae）四倍体诱导的不同休克后温度。将新受精的卵分为四组，三组在受精后 24 分钟（mpf）进行热休克（40°C 2 分钟），另一组不进行休克（对照组）。接受温度休克的组进一步在以下温度下分离：22°C、26°C 和 28°C。计算胚胎发育过程中的存活率，并在孵化时通过流式细胞术分析ploidy。结果表明，休克后温度会影响分析的参数，因此，必须考虑优化 A. altiparanae 四倍体的生产。这些数据是创新性的，可以用于该物种未来的基础生物学研究。
