Zhang Shichao, Zeng Zhu, Liu Yongfen, Huang Jiangtao, Long Jinhua, Wang Yun, Peng Xiaoyan, Hu Zuquan, Ouyang Yan
Immune Cells and Antibody Engineering Research Center of Guizhou Province, Key Laboratory of Biology and Medical Engineering, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, Guizhou, P.R. China.
Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, Guizhou, P.R. China.
Aging (Albany NY). 2020 Sep 23;12(18):17958-17975. doi: 10.18632/aging.103519.
The tumor microenvironment is closely related to the progression and immune escape of tumor cells. Tumor-infiltrating immune cells (TIICs) and immune-related genes (IRGs) are indispensable components of the tumor microenvironment and have been demonstrated to be highly valuable in determining the prognosis of multiple cancers. To elucidate the prognostic value of TIICs and IRGs in gastric cancer, we conducted a comprehensive analysis focusing on the abundances of 22 types of TIICs and differentially expressed IRGs based on a dataset from The Cancer Genome Atlas (TCGA). The results showed that great composition differences in TIICs and immune cell subfractions were associated with survival outcomes in different stages. Additionally, 29 hub genes were characterized from 345 differentially expressed IRGs and found to be significantly associated with survival outcomes. Then, an independent prognostic indicator based on ten IRGs was successfully constructed after multivariate adjustment for some clinical parameters. Further validation revealed that these hub IRGs could reflect the infiltration levels of immune cells. Thus, our results confirmed the clinical significance of TIICs and IRGs in gastric cancer and may establish a foundation for further exploring immune cell and gene targets for personalized treatment.
肿瘤微环境与肿瘤细胞的进展和免疫逃逸密切相关。肿瘤浸润免疫细胞（TIICs）和免疫相关基因（IRGs）是肿瘤微环境不可或缺的组成部分，并且已被证明在确定多种癌症的预后方面具有很高的价值。为了阐明TIICs和IRGs在胃癌中的预后价值，我们基于来自癌症基因组图谱（TCGA）的数据集，对22种TIICs的丰度和差异表达的IRGs进行了全面分析。结果表明，TIICs和免疫细胞亚群的巨大组成差异与不同阶段的生存结果相关。此外，从345个差异表达的IRGs中鉴定出29个枢纽基因，并发现它们与生存结果显著相关。然后，在对一些临床参数进行多变量调整后，成功构建了一个基于10个IRGs的独立预后指标。进一步验证表明，这些枢纽IRGs可以反映免疫细胞的浸润水平。因此，我们的结果证实了TIICs和IRGs在胃癌中的临床意义，并可能为进一步探索免疫细胞和基因靶点以进行个性化治疗奠定基础。
